성견에서 농축 성장 인자와 혈소판 풍부 섬유소의 조기 골형성 능력의 비교

Abstract

Purpose : The purpose of this study was to compare the predictability of new bone formation in peri-implant defect at the femur of the adult dog using an autologous concentrated growth factors (CGF) graft alone and platelet graft alone as alternative graft material. Material and Methods : Platelet rich fibrin(PRF) and concentrated growth factors(CGF) was made using venous blood from the jugular vein of 6 adult dogs. After infiltrative anesthetizing to right femur bone of an adult dog, 8 mm sized 4 bony defects were formed and 3.7x10mm implants were placed. PRF, CGF and allograft material were grafted to bony defect area and had 2 weeks of healing period. After healing period the experiment was conducted to left femur bone with same methods. 2 weeks later, experimental animals were sacrified and histological examination were performed. Formed PRF and CGF were stored in small bowl then plasma was collected andELISAquantitative analysis was performed with that plasma to detect containing typical growth factor such as TGF-β and VEGF. And microscopic analysis of fibrinogen structure was done using dehydrated sample of PRF and CGF. Result : In case of the new bone formation rate at histological examination using optical microscope, there was not statistical significance at 2 weeks(P=.214) experimental group while statistically significant new bone formation rate was shown at 4 weeks experimental group(P=.002). Among comparision of each 4 weeks experimental group there was statistical significance at CGF group(P=.034) and allobone graft material group(P=.023). When comparing CGF groug and allograft material group, allograft material group showed much more new bone formation significantly. In case of bone to implant contact rate, increasing rate at 2 weeks experimental group was not significant(P=0.166) while 4 weeks experimental group showed significant increment(P=0.028). At comparison between 4 weeks groups, allograft material group had statistical significance(P=0.028). Using ELISA, TGF-β was so extremely few thus it was failed to detect. In case of VEGF, CGF had VEGF as much as 1.5 times than PRF but statistical significance wasn't shown(p=.177). The diameter of fibrinogen per unit area was compared using scanning electromicroscope(SEM) and CGF showed fibrinogen structure with constant diameter. On the other hand, PRF showed fibrinogen structure with irregular diameter per unit area. Conclusion : In comparison CGF with PRF graft to bony defect area, in case of CGF much more new bone formation was observed and although there wasn't statistical significance, much more VEGF content was shown than PRF. When applying to clinical case, it is predicted that CGF would show better result than PRF. But new bone formation was not observed as much as enough to be alternative for bone graft material, thus it is thought to need further studies.