전기 유체역학 공정을 이용한 조직공학용 바이오리액터 개발

Abstract

In general, various physical stimulations have been widely applied to tissue regenerative applications. In particular, for bone tissue regeneration several experiments reported the electric stimulation can enhance the mineral formation in cultured osteoblasts and even alter the pattern of gene expression promoting in bone tissue formation. However, to date, for rapid-prototyped PCL composites consisted of pure PCL and dispersed material including single wall carbon nanotubes (SWCNT) and β-tricalcium phosphate (β-TCP), the effect of the electric stimulation on various cellular activities has not been analyzed. Here, a sinusoidal AC electric field (55 ± 8 mV/cm and 60 Hz) between parallel electrodes was applied to the 3 dimensional (3D) scaffolds (pure PCL, PCL/SWCNT 0.2 wt%, PCL/β-TCP 20 wt%) cultured with osteteoblast-like-cells (MG-63) with every 30 min/day for 14 days. For all scaffolds, when exposed with the electric stimulation, the ALP and calcium mineralization for all scaffolds were enhanced, but the PCL/β-TCP scaffold showed the highest improvement of bone mineralization compared to those of other scaffolds. In this work, we cannot completely explain about the phenomenon, but we can estimate that the improvement can be due to the calcium ions chemically precipitated from the PCL/β-TCP scaffolds. To evaluate the effect of the released calcium ions, we measured the change of physical shape of proliferated cells under the electric stimulation. The result can mean that not only the applied electric field conditions can be important parameters for electric stimulation, but also the scaffold consisting materials are another important parameter to successful electric stimulation.