660 nm 광 조사가 치은섬유모세포 (HGF) 및 조골모세포(MC3T3-E1)에 미치는 영향

Abstract

Photobiomodulation by light in the red to near infrared range (630–1000 nm) using light-emitting diode (LED) arrays has been shown to accelerate wound healing, improve recovery from ischemic injury in the heart and attenuate degeneration in the injured optic nerve. In this study, we have fabricated the 660 nm red LED illumination controller and LED array module and evaluated the proliferation and differentiation of preosteoblast MC3T3-E1 cells and cell migration of human gingival fibroblast (HGF) cells under different light intensity and illumination time. To investigate the 660 nm LED effects on MC3T3-E1 and HGF cells, cells were exposed to LED irradiation at illumination time of 5, 10, 15, 20 min and light intensity of 8.5 mW/cm2. The proliferation and differentiation of MC3T3-E1 cells were evaluated by MTT assay and Alkaline phosphatase (ALP) activity, respectively. In addition, cell migration of HGF cells was investigated by two-dimensional migration assay and three-dimensional transwell migration assay. According to the 660 nm LED irradiation experiment results are as follows. 1) Exposure to LED light intensity of 8.5 mW/cm2 and illumination time of 5 min significantly increased MC3T3-E1 cells proliferation and cell differentiation, suggesting that 660 nm LED stimulates cell in a time-dependent. 2) The number of migrated HGF cells showed the largest increase in experimental group under LED light intensity of 8.5 mW/cm2 and illumination time of 5 min. 3) The migration rate of HGF cells showed the fastest in experimental group for culturing time of 12 hr after LED light intensity of 8.5 mW/cm2 and illumination time of 5 min.

These data that suggest 660 nm red light exposure may be helpful in postoperative wound repair of HGF cells and proliferation and differentiation of MC3T3-E1 cells.