항균물질이 포함된 분해성고분자의 전기방사에 의한 나노섬유의 제조 및 항균성 평가

Metadata Downloads
Issued Date
Electrospinning is a handy and cost-effective technique for producing nanowebs where the fiber diameters are in the range of a few hundred nanometers to a few microns. The technique is very promising and versatile since it facilitates the production of multifunctional nanofibers from various polymers, polymer blends, sol gels, composites and ceramics, etc.1~4)
We made fiber out of the polymer solution which added silver, ampicillin, streptomycin and herb extracts to poly lacticacid / (Methylene Chloride/Ethyl alcohol)(80/20) solution through the electrospinning. Electrospinning conditions was as fallows ; 100㎕/min of flow rate, and 10cm of tip - to - collector distance(TCD), applied voltage was 15kV. After electrospinning precess, fiber morphology was observed from Scanning Electron Microscope (SEM).
Antimicrobial activities of poly lacticacid fiber from added silver, ampicillin, streptomycin and herb extracts was investigated by disc diffusion test and optical density test, colony counting test and vapor diffusion test against E. coll and S. aureus. In case of antimicrobial activities of E. coli and S. aureus, the clean zone diameter was increase according to content of antimicrobial additive.
The average diameters of PLA nanofibers containing from 0.1 wt% to 0.5 wt% of AgNO3, ampicillin and streptomycin were from 1905nm to 799nm, from 2386nm to 1287nm and from 1802nm to 840nm. Bacteria reduction of silver - PLA nanofiber, ampicillin - PLA nanofiber and streptomycin - PLA nanofiber was 0.5 wt% silver- PLA nanofiber > 0.5 wt% streptomycin - PLA nanofiber > 0.5 wt% ampicillin - PLA nanofiber for S. aureus by optical density test during 24 hours. Electrospun PLA nanofibers containing 0.5 wt% of silver showed 100% bacterial activity against Staphylococcus aureus and Escherichia coli after 8 hours by colony counting test.
Alternative Title
Preparation and Evaluation of Electrospun nanofibers for biodegradable polymer containing antibiotic materials.
Alternative Author(s)
Kim Mi Jin
조선대학교 환경공학과
일반대학원 환경공학
Awarded Date
Table Of Contents
Table of Contents Ⅰ
List of Tables Ⅳ
List of Figures Ⅴ
Abstract Ⅸ

1. 서론 1

2. 이론적 고찰 3
2.1. 전기방사(electrospun) 3
2.2. PLA(polylactic acid) 5
2.3. 합성항균제 7
2.4. 허브추출물 8

3. 재료 및 실험 방법 9
3.1. 실험 재료 9
3.1.1. 재료 9
3.1.2. 항균활성 검색용 균주 및 배지 9

3.2. 실험 방법 11
3.2.1. 방사용액 제조 11
3.2.2. 방사용액의 물성 측정 11
3.2.3. 실험장치 12
3.2.4. 형태학적 특성 측정 12

3.3. 항균활성 측정 14
3.3.1. 항균활성 측정 방법 14
가) Disc diffusion test
나) Optical density test
다) Colony counting test
라) Vapour diffusion test

4. 결과 및 고찰 16
4.1. 합성항생제 16
4.1.1. 형태학적 특성 고찰 16
4.1.2. Disc diffusion test 21
4.1.3. Optical density test 27
4.1.4. Colony counting test 42
4.1.5. MIC 45

4.2. 허브추출물 58
4.2.1. Vapour diffusion test 58
4.2.2. MIC 61

5. 결론 58
Reference 66
김미진. (2009). 항균물질이 포함된 분해성고분자의 전기방사에 의한 나노섬유의 제조 및 항균성 평가.
Appears in Collections:
General Graduate School > 3. Theses(Master)
Authorize & License
  • AuthorizeOpen
  • Embargo2010-01-25
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.