항균물질이 포함된 분해성고분자의 전기방사에 의한 나노섬유의 제조 및 항균성 평가

Abstract

Electrospinning is a handy and cost-effective technique for producing nanowebs where the fiber diameters are in the range of a few hundred nanometers to a few microns. The technique is very promising and versatile since it facilitates the production of multifunctional nanofibers from various polymers, polymer blends, sol gels, composites and ceramics, etc.1~4) We made fiber out of the polymer solution which added silver, ampicillin, streptomycin and herb extracts to poly lacticacid / (Methylene Chloride/Ethyl alcohol)(80/20) solution through the electrospinning. Electrospinning conditions was as fallows ; 100㎕/min of flow rate, and 10cm of tip - to - collector distance(TCD), applied voltage was 15kV. After electrospinning precess, fiber morphology was observed from Scanning Electron Microscope (SEM). Antimicrobial activities of poly lacticacid fiber from added silver, ampicillin, streptomycin and herb extracts was investigated by disc diffusion test and optical density test, colony counting test and vapor diffusion test against E. coll and S. aureus. In case of antimicrobial activities of E. coli and S. aureus, the clean zone diameter was increase according to content of antimicrobial additive. The average diameters of PLA nanofibers containing from 0.1 wt% to 0.5 wt% of AgNO3, ampicillin and streptomycin were from 1905nm to 799nm, from 2386nm to 1287nm and from 1802nm to 840nm. Bacteria reduction of silver - PLA nanofiber, ampicillin - PLA nanofiber and streptomycin - PLA nanofiber was 0.5 wt% silver- PLA nanofiber > 0.5 wt% streptomycin - PLA nanofiber > 0.5 wt% ampicillin - PLA nanofiber for S. aureus by optical density test during 24 hours. Electrospun PLA nanofibers containing 0.5 wt% of silver showed 100% bacterial activity against Staphylococcus aureus and Escherichia coli after 8 hours by colony counting test.