생리식염수 세척이 구강소독제에 의한 타액 내 세균 수의 변화에 미치는 영향

Abstract

Numberless bacteria live in the oral cavity and a number of mouthwashes are available to antiseptic agents which differ in regimen of use. Nevertheless, complete sterilizing disinfectants of oral cavity has not been developed yet. There are some differences between each clinics about oral antiseptic agents and methods used in oral and maxillofacial surgeries. The aim of the present study was to investigate the effect of saline irrigation in combination with mouthwash of antiseptic agents on salivary bacterial counts. This study was performed with a randomized study on employing a panel of 80 young healthy human volunteers (40 males and 40 females) between the age of 26 and 32 years. Volunteers were randomized to one of eight treatment groups. In the first test, 0.2ml of non-stimulatory saliva was collected from every subjective person. Then, 0.2ml of saliva after rinsing for 30 seconds or 60 seconds with chlorhexidine and povidone-iodine solutions was collected from 40 people respectively. In the second test, fifth to eighth groups rinsed for 30 seconds or 60 seconds with chlorhexidine and povidone-iodine solution respectively. And then 0.2ml saliva was collected after irrigation with normal saline. These samples were cultured for 48 hours aerobically. The colony-forming units (CFU) were calculated at each time point and reduction rates of CFU, that cultured before and after saline irrigation, between two groups with the identical agent and application time were tested using student t-test. Chlorhexidine showed significant decrease on CFU when being applied for 1 minute in combination with saline irrigation (p<0.01). And povidone-iodine showed significant decrease of CFU when applied for 30 seconds compared with chlorhexidine applied for 30 seconds (p<0.01). It was concluded that chlorhexidine and povidone-iodine were effective oral antiseptic agents. Especially, when chlorhexidine was used in combination with saline irrigation, salivary bacterial counts were significantly decreased.