고농도 산소수에 의한 새싹식물의 항산화 물질 증가 효과

Abstract

This study was carried out to investigate the effects of the antioxidant activities on the dissolved oxygen concentration and UV treatment for red radish, broccoli, kale, red kale sprouts. The dissolved oxygen concentration was controlled by pure oxygen and bubble column for various concentration such as 8 mg/L O₂, 20 mg/L O₂, 30 mg/L O₂. Extracts of sprouts were prepared from 10g of grinded sprouts putting in 60% ethanol in the water at 60℃ for 4hours. Total poly phenol contents were measured by the Folin-Denis method and total flavonoid contents by transformed Davis method. Based on Blois method, radical scavenging activities of sprout extracts were examined by using DPPH (1,1-diphenyl-2-picrylhydrazyl). As dissolved oxygen concentration increase, the initial germination and growth of sprouts was increased. The total poly phenol contents of broccoli was modified to 17092.2 mg/100g at the conditions for 30 mg/L O₂ of dissolved oxygen as the highest concentration. Radical scavenging ability of sprouts was ordered broccoli sprout > red radish sprout > kale sprout > red kale sprout and experiment of conditions of radical scavenging with broccoli sprout was the highest as 30 mg/L. In order to examining the effect of antimicrobial materials on the germination and growth for sprouts, 0.2% ampicillin, 0.2% H₂O₂, 2% thyme(hub extract oil) added culture solution to reduce the microorganism numbers. In case of 0.2% ampicillin and 0.2% H₂O₂, gemination and growth of red radish sprout were not influenced by antimicrobial materials and red radish sprout was not germinated for 2% thyme.