아트로핀 근주를 이용한 단기간 건성안 동물모델

Abstract

Abstract

A Short-Term Dry Eye Animal Model by Intramuscular Injection of Atropine

Seong-Won Yang Advisor : Nam-Cheol Ji M.D.,PhD. Department of Medicine Graduate School of Chosun University

Purposes: To figure out if we could use a short term model of dry eye by an intramuscular injection of atropine to rabbits, and the estimation of tear secretion amount and the evaluation of the corneal and conjunctival changes

Subjects and Methods: Among 10 rabbits, five of them were injected 1 mg/kg of 1% atropine sulfate everyday, and other five of them as control group were injected 1 mg/kg of normal saline. Then, Schirmer test was performed to estimate the ratio of tear secretion before and after atropine injection. After 1% fluorescence dye was instilled, the degree of dying was observed by using slit lamp, then divided into grades by staining of cornea. In 10 days, impression cytology test was used to measure goblet cells for all rabbits.

Results: Schirmer test was performed for 10 days. It was done after every atropine injection. After two days, the ratio of tear secretion amount was significantly decreased. The staining of cornea was graded from 0 to 1 before the atropine injection, but it was still graded from 0 to 1 in 10 days. The significant difference was not observed between the experimental and control groups. The number of average goblet cells was 14.02±4.447 (cells/㎟) in experimental group which was injected with the atropine, and 43.32±7.531 (cells/㎟) in the control group which was injected with the normal saline. The significant difference was found between the two groups (P<0.05).

Conclusions : A Significant difference was observed in association with the ratio of tear secretion, but atropine does not affects the fluorescence grade of cornea significantly. At the impression cytology test, the number of average goblet cell was changed significantly in contrast to the control group.