구강 내 임플란트-지대주 연결부에서 치주질환관련 세균의 검출

Abstract

Statement of the problem: Implant systems result in gaps and cavities between implant and abutment that can act as a trap for bacteria and thus possibly cause inflammatory reactions in the peri-implant soft tissues. Purpose: The purpose of this study was to investigate the detection rate of putative periodontopathogens, Porphyromonas. gingivalitis, Prevotella. intermedius, Tannerella. forsythensis, Treponema. denticola, Actinobacillus. actionmycetemcomitans, related to implant-abutment interface microleakage. Material and methods: Samples were taken from 27 subjects with sterilized paper points and were transported in 1*PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced in order to confirm the specificity. Results: Our data showed that the detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant fixture was 59% and 82% in patients respectively. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant crevice was 44% and 82% in patients. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in tongue was 82% and 82% in patients. Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.