양성, 전암성, 암성 각질형성세포 과증식성 병변에서의 Ki-67, p53, p63, EGFR 단백 발현에 대한 분석

Abstract

The keratinocytic hyperproliferative lesions encompass a wide range of non-cancerous, precancerous and cancerous conditions. The purpose of this study was to investigate the expression of EGFR, Ki-67, p53 and p63 in this lesion. Immunoperoxidase staining methods were applied to analyze p53, p63, Ki-67 protein and EGFR expression in a total of 58 cases, including 7 keratoacanthomas, 7 seborrheic keratoses, 11 actinic keratoses, 8 Bowen's disease and 25 squamous cell carcinomas. There was a statistically significant increase in p53, p63, Ki-67 staining of SCCs compared with normal seborrheic keratoses. Actinic keratoses showed a trend towards increased expression in the basal layer compared with normal skin. The pattern of expression showed diffuse staining in Bowen's disease and SCCs(3+ ~ 4+). In actinic keratoses, Keratoacanthomas and seborrheic keratoses the pattern was most prominent in the basal layer. EGFR had a strong membranous expression in all specimens. In actinic keratoses the pattern was most prominent in the basal layer(1+). p53, p63 and Ki-67 expressions were greater in Bowen's disease and SCCs compared with normal skin, actinic keratoses, seborrheic keratoses or keratoacanthomas. The expressions of actinic keratoses, seborrheic keratoses, and keratoacanthomas were mostly restricted to the basal layer. Although significant diffuse staining was also noted. EGFR was overexpressed in all samples of skin lesion. The expression level of p53 had a significantly positive relation with the expression level of p63. The expression level of p63 had a significantly positive relation with the expression level of Ki-67. Our results support that EGFR, p53, p63 and Ki-67 play a role in cell differentiation & tumorigenesis of skin lesion.