유근피 추출물이 흰쥐의 항산화계 및 지질대사에 미치는 영향
- Author(s)
- 정창주
- Issued Date
- 2006
- Abstract
- The results obtained were as follows :
1.In U. davuidian var. japonica, fructose was major in free sugars, aspartic acid in amino acids, palmitic acid in fatty acids, formic acid in organic acids, calcium in minerals, Ca++ in cations and NO3- in anions, respectively.
2.On antioxidative activity of UDEE in vitro, n-butanol fraction was the highest among fractions, and activity of nitrite scavenging was the highest at pH 1.2, and the peroxide value for linoleic acid was increased during the storage.
3.In scavenging effect of oxygen free radicals generated in the ethanol- induced hepatotoxicity in the liver of rats,
① The growth rate of the rats was decreased by ethanol administration, however, was gradually increased to a little lower level than the normal group by administering UDEE.
② It was also observed that the activities of superoxide dismutase(SOD) increased by ethanol were markedly decreased in UDEE administered group as compared with control group. The glutathione(GSH) contents were decreased by ethanol adminstration, however, increased after administering UDEE.
4.In antihypercholesterolemic effect of UDEE on the levels of lipid in serum and the liver of rats fed high cholesterol diet,
① The growth rate of the hypercholesterolemic group(control group) was higher than the normal group, whereas the groups administered UDEE were decreased. The liver weight in control group was significantly higher than that of normal group, but that of control group with UDEE administration was lower than control group.
② The activities of alanine aminotransferase(ALT), asparate amino- transferase(AST) and alkaline phosphatase(ALP) in serum were rather lowered in the UDEE administration group than in the cholesterol administered group.
③ Contents of total cholesterol and LDL-cholesterol of in serum were lower in UDEE administration groups than control group while those of HDL-cholesterol were higher.
UDEE increased HDL-C/T-C ratio and lowered atherogenic index. Triglyceride content was not decreased significantly during 6 weeks of experiment, but the contents of free cholesterol and cholesteryl ester were significantly decreased in UDEE administrated groups than control group. The phospholipid content in serum was not affected significantly by UDEE, but it was gradually increased. The hepatic contents of total cholesterol and triglyceride were lowered in UDEE administrated groups than control group.
These results suggest that ethanol extract of U. davuidian var. japonica have a possible positive effect on the liver function in hepatotoxicity-induced rats by ethanol administration, and the fatty liver in hyperlipidemia-induced rats by high cholesterol administration.
- Alternative Title
- Effect of Ulmus davidiana var, japonica Nakai Ethanol Extract on Antioxidative System and Lipid Metabolism of Rats
- Alternative Author(s)
- Jung, Chang-Joo
- Affiliation
- 조선대학교 대학원
- Department
- 일반대학원 식품영양학과
- Advisor
- 이명열
- Awarded Date
- 2006-02
- Table Of Contents
- ABSTRACT = ⅹ
제1장 서론 = 1
제2장 재료 및 방법 = 6
제1절 실험재료 = 6
제2절 성분분석 = 6
1.일반성분 = 6
2.유리당 = 6
3.아미노산 = 7
4.지방산 = 8
5.비타민 A 와 비타민 E = 9
6.유기산 = 10
7.무기질 = 11
8.양.음이온 = 12
제3절 항산화 효능 실험 = 14
1.시약 = 14
2.실험기기 = 15
3.시료추출 = 15
가.에탄올 추출 = 15
나.다용매 분획 추출 = 16
4.In vitro 에서 항산화활성 = 18
가.DPPH radical소거작용 = 18
나.항산화지수(activeoxygenmethod(AOM)-Rancimat측정) = 18
다.아질산염 소거 작용(nitritescavengingability,NSA) = 19
라.Linoleicacid에 대한 항산화 작용 = 19
마.지질과산화물 생성 억제 작용(TBARS) = 20
5.In vivo 에서 항산화효능 = 20
가.실험동물 = 20
나.에탄올추출물 및 알코올 투여 = 21
다.체중 및 식이섭취량 = 2
라.실험동물 처치 = 21
마.효소활성 = 23
(1)효소원 조제 = 23
(2)XO 활성 = 23
(3)SOD 활성 = 24
(4)Catalase활성 = 24
(5)GSH-Px활성 = 24
(6)TBARS함량 = 25
(7)GSH 함량 = 25
(8)단백질 정량 = 26
바.혈청 중의 AST,ALT,ALP 활성 = 26
사.통계처리 = 26
제4절 고지혈증 억제효능 실험 = 27
1.In vitro 에서 고지혈증 억제 효과 측정 = 27
가.LDL의 분리 = 27
나.LDL 산화 억제 효과 = 27
2.In vivo 에서 고지혈증 억제효능 실험 = 28
가.실험동물의 사육 및 식이 = 28
나.실험동물의 처리 = 30
다.혈청 중 효소활성 = 30
라.혈청 중 지질함량 = 31
마.간장 중 지질함량 = 31
제3장 결과 및 고찰 = 32
제1절 성분분석 = 32
1.일반성분 = 32
2.유리당 = 33
3.구성아미노산 조성 = 33
4.지방산 조성 = 34
5.비타민 A 및 E = 36
6.유기산 = 36
7.무기질 = 37
8.양.음이온의 = 37
제2절 항산화 효능 = 39
1.In vitro 에서 유근피 에탄올 분획의 항산화 활성 = 39
가.DPPH radical소거작용 = 39
나.항산화지수(AOM) = 41
다.아질산염 소거작용 = 43
라.지질과산화에 생성억제 작용 = 43
마.Linoleicacid에 대한 항산화 작용 = 45
2.In vivo 에서 유근피 에탄올 추출물의 항산화 효능 = 46
가.체중증가율 및 식이효율 = 46
나.간 조직 중 항산화 효소 활성 = 49
(1)유리기 생성계 효소(XO)활성 = 49
(2)유리기 해독계 효소(SOD,catalase및 GSH-Px)활성 = 51
다.간 조직 중 과산화지질 함량 = 55
라.간 조직 중 GSH함량 = 57
마.혈청 중 ALT,AST 및 ALP 활성 = 59
제3절 고지혈증 억제 효능 = 63
1.In vitro 에서 고지혈증억제 효과 = 63
가.LDL에 대한 항산화 효과 = 63
2.In vivo 에서 고지혈증억제 효능 = 65
가.체중증가율,식이효율 간장/체중비율 = 65
나.혈청 중 중성지질,총콜레스테롤 및 인지질 함량 = 69
다.혈청 중 유리콜레스테롤,콜레스테릴 에스테르농도 및 = 콜레스테릴 에스테르 비율 = 73
라.혈청 중 LDL-콜레스테롤,HDL-콜레스테롤,심혈관 = 위험지수 (CRF)및 동맥경화지수 = 77
마.혈청 중 ALT,AST 및 ALP활성 = 83
바.간장 총 콜레스테롤 및 중성지질 농도 = 87
제4장 요약 = 91
제5장 참고문헌 = 94
- Degree
- Doctor
- Publisher
- 조선대학교 대학원
- Citation
- 정창주. (2006). 유근피 추출물이 흰쥐의 항산화계 및 지질대사에 미치는 영향.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/6206
http://chosun.dcollection.net/common/orgView/200000233061
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