삼백초와 죽엽 추출물의 항산화 및 모유두세포 증식효과

Abstract

With different methods to extract active ingredients from Saururus chinensis and Phyllostachys Folium in order to measure antioxidative effect, this study confirms cellulotoxic property and cell proliferation of human hair papilla with MIT assay by selecting extracts with the best method of top DPPH elimination effect, while aiming to provide practical materials for development of functional cosmetics as well as identifying potential of hair growth and prevention of hair loss with natural substances that replace current pharmacotherapy through the study on protein manifestation facilitation effect with western blotting. Total polyphenol content of Saururus chinensis and Phyllostachys Foliumnd turned out to be the highest at 76.588 mg/g from methanol-ultrasonic wave extracts of Saururus chinensis and 70.616 mg/g from methanol extracts of Phyllostachys Folium. Total flavonoid content turned out to be the highest at 19.321 mg/g from methanol-ultrasonic wave extracts of Saururus chinensis while methanol-ultrasonic wave extracts of Phyllostachys Folium had 9.153 mg/g. The highest DPPH elimination effect turned out to be 92.81% (methanol-ultrasonic wave extracts of Saururus chinensis) and 49.90% (methanol-ultrasonic wave extracts of Phyllostachys Foliumnd), with Saururus chinensis having stronger effect than Phyllostachys Folium. Also highest nitrite elimination effects were 36.90% from methanol-ultrasonic wave extracts of Saururus chinensis and 31.55% from methanol-ultrasonic wave extracts of Phyllostachys Folium, which were lower than 90 ~ 93% of ascorbic acid (1 mg/ml) used as positive control group. Upon checking cellulotoxic property on human hair papilla with MTT assay by applying methanol-ultrasonic wave extracts of each substance with content of 10 μg/ml, 100 μg/ml, and 500 μg/ml, both turned out not to have meaningful cellulotoxic property within treatment concentration. Regarding hair papilla growth, treatment with Saururus chinensis extracts of 10 μg/ml showed 93.9%, while 96.2% at 100 μg/ml and 121.6% at 500 μg/ml were the results. Treatment with Phyllostachys Folium extracts of 10 μg/ml showed 93.6%, while 99.1% at 100 μg/ml and 90.8% at 500 μg/ml were the results, which represented higher degree of growth along with stronger concentration. Measurement of PDGF-B growth factor manifestation facilitation effect through western blotting resulted in 98% for Saururus chinensis extracts against the control group with PBS treatment and 96% for Phyllostachys Folium extracts, while IGF growth factor manifestation effects were 106% for Saururus chinensis extracts and 98% for Phyllostachys Folium extracts, meaning higher effects for Saururus chinensis extracts. VEGF manifestation facilitation effects were 97% for Saururus chinensis extracts and 95% for Phyllostachys Folium extracts, with no difference than the control group. In case of KGF, Saururus chinensis extracts showed 97% and Phyllostachys Folium extracts showed 102% with no difference than the control group. Also, in case of hGH, Saururus chinensis extracts indicated 96% and Phyllostachys Folium extracts showed 103%, in EGF, Saururus chinensis extracts indicated 96% and Phyllostachys Folium extracts indicated 1 03%. So, there is no difference from the control group, This study has found that Saururus chinensis extracts have values as the ingredient for treatment to facilitate hair growth and development of a hair growth promoter as it had better performance of DPPH elimination effect.

Keyword: Hair loss, Saururus Chinensis, Bamboo leaf, Antioxidative property, Western blott, Growth factor