삼백초와 죽엽 추출물의 항산화 및 모유두세포 증식효과

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모유두세포에 대한 삼백초추출물과 죽엽추출물의 항산화 및 성장인자 발현촉진효과
With different methods to extract active ingredients from Saururus chinensis and Phyllostachys Folium in order to measure antioxidative effect, this study confirms cellulotoxic property and cell proliferation of human hair papilla with MIT assay by selecting extracts with the best method of top DPPH elimination effect, while aiming to provide practical materials for development of functional cosmetics as well as identifying potential of hair growth and prevention of hair loss with natural substances that replace current pharmacotherapy through the study on protein manifestation facilitation effect with western blotting.
Total polyphenol content of Saururus chinensis and Phyllostachys Foliumnd turned out to be the highest at 76.588 mg/g from methanol-ultrasonic wave extracts of Saururus chinensis and 70.616 mg/g from methanol extracts of Phyllostachys Folium. Total flavonoid content turned out to be the highest at 19.321 mg/g from methanol-ultrasonic wave extracts of Saururus chinensis while methanol-ultrasonic wave extracts of Phyllostachys Folium had 9.153 mg/g.
The highest DPPH elimination effect turned out to be 92.81% (methanol-ultrasonic wave extracts of Saururus chinensis) and 49.90% (methanol-ultrasonic wave extracts of Phyllostachys Foliumnd), with Saururus chinensis having stronger effect than Phyllostachys Folium. Also highest nitrite elimination effects were 36.90% from methanol-ultrasonic wave extracts of Saururus chinensis and 31.55% from methanol-ultrasonic wave extracts of Phyllostachys Folium, which were lower than 90 ~ 93% of ascorbic acid (1 mg/ml) used as positive control group.
Upon checking cellulotoxic property on human hair papilla with MTT assay by applying methanol-ultrasonic wave extracts of each substance with content of 10 μg/ml, 100 μg/ml, and 500 μg/ml, both turned out not to have meaningful cellulotoxic property within treatment concentration. Regarding hair papilla growth, treatment with Saururus chinensis extracts of 10 μg/ml showed 93.9%, while 96.2% at 100 μg/ml and 121.6% at 500 μg/ml were the results. Treatment with Phyllostachys Folium extracts of 10 μg/ml showed 93.6%, while 99.1% at 100 μg/ml and 90.8% at 500 μg/ml were the results, which represented higher degree of growth along with stronger concentration.
Measurement of PDGF-B growth factor manifestation facilitation effect through western blotting resulted in 98% for Saururus chinensis extracts against the control group with PBS treatment and 96% for Phyllostachys Folium extracts, while IGF growth factor manifestation effects were 106% for Saururus chinensis extracts and 98% for Phyllostachys Folium extracts, meaning higher effects for Saururus chinensis extracts. VEGF manifestation facilitation effects were 97% for Saururus chinensis extracts and 95% for Phyllostachys Folium extracts, with no difference than the control group. In case of KGF, Saururus chinensis extracts showed 97% and Phyllostachys Folium extracts showed 102% with no difference than the control group. Also, in case of hGH, Saururus chinensis extracts indicated 96% and Phyllostachys Folium extracts showed 103%, in EGF, Saururus chinensis extracts indicated 96% and Phyllostachys Folium extracts indicated 1 03%. So, there is no difference from the control group,
This study has found that Saururus chinensis extracts have values as the ingredient for treatment to facilitate hair growth and development of a hair growth promoter as it had better performance of DPPH elimination effect.

Keyword: Hair loss, Saururus Chinensis, Bamboo leaf, Antioxidative property, Western blott, Growth factor
Alternative Title
Antioxidative and Proliferative Effects of Follicle Dermal Papilla Cell of Extracts from Saururus chinensis and Phyllostachys Folium
Alternative Author(s)
Park gum dan
일반대학원 보완대체의학과
Awarded Date
Table Of Contents
목 차

표 목차 ···············································································ⅲ
그림 목차 ···········································································ⅸ
ABSTRACT ········································································ⅷ

Ⅰ. 서론··················································································1
1. 연구의 배경 및 필요성 ······················································1
2. 연구의 목적······································································5

Ⅱ. 연구방법··········································································6
1. 재료 및 시약·····································································6
2. 추출물의 제조···································································6
1) 유기용매 추출································································6
2) 증류수 추출···································································7
3) 초임계 추출···································································8
3. 항산화 효과 분석·····························································10
1) 총 폴리페놀 함량 측정····················································10
2) 총 플라보노이드 함량 측정··············································10
3) DPPH 라디칼 소거능 측정···············································10
4) 아질산염 소거능 측정 소거능 측정·····································11
4. 인간 모유두세포에 대한 in vitro 세포 독성 평가··················12
1) 천연추출물 처리····························································12
2) 세포배양·····································································12
3) MTT assay································································12
5. Western blotting을 통한 성장인자 발현에 미치는 영향··········14
6. 통계처리·········································································15

Ⅲ. 연구결과·········································································16
1. 생약제재 추출물의 수율····················································16
2. 항산화 효과····································································17
1) 총 폴리페놀 함량··························································17
2) 총 플라보노이드 함량·····················································19
3) DPPH 라디칼 소거능······················································21
4) 아질산염 소거능····························································23
3. 인간 모유두세포에 대한 invitro 세포 독성 평가····················25
1) 삼백초········································································25
2) 죽엽···········································································26
3) 삼백초 추출물과 죽엽 추출물의 1:1 혼합물··························27
4. 성장인자 발현 촉진 효과···················································28
1) PDGF-B 발현 촉진 효과··················································28
2) IGF 발현 촉진 효과·······················································29
3) VEGF 발현 촉진 효과·····················································30
4) KGF의 발현 촉진 효과····················································31
5) hGH 발현 촉진 효과······················································32
6) EGF 발현 촉진 효과·······················································33

Ⅳ. 고찰················································································34
Ⅴ. 결론················································································40

박금단. (2016). 삼백초와 죽엽 추출물의 항산화 및 모유두세포 증식효과.
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