녹나무와 동백 씨 추출물 유래 기능성화장품 소재 개발

Abstract

This study has been conducted with the aim of providing practical materials for development of an anti-hair loss agent and hair growth promotor that utilizes natural ingredients replacing medications through experimental methods testing growth factor protein expression promotion effect, based on MTT assay and Western blotting on human hair papilla cells and antioxidative capacity by extracting active ingredients in 6 methods such as hot water extraction, ethanol extraction, methanol extraction, ethanol-ultrasonic wave extraction, methanol-ultrasonic wave extraction and superficial extraction from the seeds of Cinnamomum camphora and Camellia japonica. The results show that total polyphenol content was the highest on the ethanol-ultrasonic wave extracts of Cinnamomum camphora and methanol extracts of Camellia japonica at 50.248 mg/g and 99.263 mg/g respectively, while total flavonoid content was the highest on Cinnamomum camphora and Camellia japonica at 5.907 mg/g and 11.811 mg/g respectively. DPPH elimination performance analysis results were that Cinnamomum camphora had the highest performance of 96.34% on methanol-ultrasonic wave extracts while Camellia japonica had the highest performance of 49.33% on superficial extracts. Nitrite elimination performances of both Cinnamomum camphora and Camellia japonica turned out as 37.59% and 37.76% respectively, which were lower than the nitrite elimination performance 90~93% of ascorbic acid (1 mg/mL), the positive control group. There was no cell toxicity of Cinnamomum camphora observed on human hair papilla cells at treatment concentrations of 10 ug/mL, 100 ug/mL and 500 ug/mL, while cell growth degree was 112.7% at 500 ug/mL. Camellia japonica seed extracts turned out to have cell toxicity at all three treatment concentrations of 10 ug/mL, 100 ug/mL and 500 ug/mL. Cell toxicity analysis of Camellia japonica seed extracts after additional dilution and treatment at concentrations of 0.1 ug/mL, 0.5 ug/mL and 1 ug/mL showed that there was no toxicity at the concentration of 0.1 ug/mL. Growth factor expression promotion effect analysis through Western blotting showed that, after processing Cinnamomum camphora at the concentration of 100 ug/mL and processing Camellia japonica seed extracts at the concentration of 0.1 ug/mL, the former had PDGF-B had 142% and the latter had 99%, 164% and 105% for IGF, 99% and 78% for VEGF respectively, 106% and 99% for KGF, 101% and 108% for hGH respectively and 97% and 99% for EGF respectively compared to the control group (PBS). This study resulted in that, Cinnamomum camphora extracts had high DPPH elimination performance, with no toxicity on human hair papilla cells and great human hair papilla cell growth promotion effect, as well as favorable PDGF-B and IGF expression effects, all of which represent high potential to be utilized for functional cosmetics and hair growth promotor with natural antioxidative effect, while Camellia japonica seed extracts had poor DPPH elimination performance and some cell toxicity, thus it has been figured that further studies regarding utilization for functional cosmetics and hair growth promotor would be required.

Keyword: Hair loss, Cinnamomum camphora, Camellia japonica, antioxidative effect, western blotting, growth factor