가축매몰지 침출수에 대한 지구화학 및 분자생물학적 특성에 관한 연구

Abstract

The leak of leachate in animal carcass disposal is a significant issue since diseases can be easily spread to humans and other livestock. In this study, the physicochemical properties of leachate and the aerobic bacteria existence in leachate using molecular biology methods for 16 animal carcass disposals in the first stage (been buried 5 months) were analyzed. The result of leachate physicochemical analysis showed higher total coliforms, TOC NH4+ and NO3- concentration compared to the relevant literature data. Also the concentration of NH4+ and NO3- in most leachate samples appeared to exceed the Korea guideline value for drinking water. For distribution of leachate using isolation and 16S rRNA sequence analysis in the aerobic condition, high frequency of Bacillus pumilus, Lysinibacillus sphaericus and Bacillus sphaericus was observed, while there were detections of food poisoning bacteria such as Bucillus cereus. This study improves our knowledge of the fate and transport in geologic media, treatment, risk analysis on the leachate from animal carcass disposal sites.