HcRNAV109 virus like particle의 자가조립 및 숙주 특이적 살조능을 이용한 유해 미세조류 Heterocapsa circularisquama의 제어

Abstract

Harmful algal blooms (HABs) are characterized by rapid and massive accumulation of certain types of microscopic algae and HABs are linked to significant socio-economic losses and can give extensive damages to the aquatic ecosystem, drinking water sources and human health by depleting the dissolved oxygen and blocking the sunlight, or producing toxins. Recombinantly expressed and purified VLPs of Heterocapsa circiularisquama RNA virus (HcRNAV) can be used as a potent carrier for harmful algal bloom suppression. The HcRNAV strains were divided in two types; HcRNAV109 (CY-type) and HcRNAV34 (UA-type), based on their complementary strain-specific infectivity. Host specific attachment and cell destruction of HcRNAV109 VLPs against H. circularisquama strains were investigated under microscopy by encapsulating the particles with a novel algicidal substance, thiazolidinedione (TD49). The TD49 concentration was detected by HPLC analysis. The VLPs encapsidating TD49 specifically attached and destroyed the cells of H. circularisquama HY9423, which are the hosts of the HcRNAV109 strains, but not the cells of HU9433-P and HA92-1, which is known as a host of HcRNAV34 virus. These results indicate that the recombinant VLPs have considerably high host specificity and are compatible with a common viral property of host specific infection. Therefore we suggest the possibility that VLPs encapsidated algicidal agents can be used as an alternative and highly effective bio-control tool to prevent a harmful algal blooming in the sea.