할미꽃 추출물에 의한 구강편평암 KB 세포의 apoptosis 유도

Abstract

The root extract of pulsatilla koreana has been used as antibacterial and anti-inflammatory analgesic agents in traditional medicine in Korea. In the present study we investigated the proliferation effect of human oral cancer KB cell treated with pulsatilla koreana extract. We analyzed the effects of this plant on proliferation and on expression of cell apoptosis related molecules with using the KB human oral cancer cells. A cell proliferation test was performed by MTT assay on 24 well plate, the treatments of pulsatilla koreana extract dramatically reduced cell viabilities in a dose and time-dependent manner. In addition, the typical apoptosis patterns were detected with DNA fragmentation and western blot. Western blot data showed that activation of caspase-3 and -7 was increased after 24 h and 12 h treatment of pulsatilla koreana extract, respectively. We also confirmed the DNA fragmentation by treatment of pulsatilla koreana extract in KB human oral cancer cells. Therefore, these results suggested that pulsatilla korean may have beneficial effects on the proliferation of oral cancer cells through activation of apoptosis.

Key Words: Apoptosis, Oral cancer, Pulsatilla koreana