플라즈마/쾌속조형공정을 통한 조골세포 재생용 PCL/β-TCP 세포담체에 대한 연구

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β-Tricalcium phosphate (β-TCP) and collagen have been widely used to regenerate various hard tissues, but although Bioceramics and collagen have various biological advantages with respect to cellular activity, their usage has been limited due to β-TCP's inherent brittleness and low mechanical properties, along with the low shape-ability of the three-dimensional collagen.

To overcome these material deficiencies, we fabricated a new hierarchical scaffold that consisted of a melt-plotted polycaprolactone (PCL)/β-TCP composite and embedded collagen nanofibers.

The fabrication process was combined with general melt-plotting methods and electrospinning. To evaluate the capability of this hierarchical scaffold to act as a biomaterial for bone tissue regeneration, physical and biological assessments were performed.

Scanning electron microscope (SEM) micrographs of the fabricated scaffolds indicated that the β-TCP particles were uniformly embedded in PCL struts and that electrospun collagen nanofibers (diameter ) 160 nm) were well layered between the composite struts.

By accommodating the β-TCP and collagen nanofibers, the hierarchical composite scaffolds showed dramatic water-absorption ability (100% increase), increased hydrophilic properties (20%), and good mechanical properties similar to PCL/β-TCP composite.

MTT-assay and SEM images of cell-seeded scaffolds showed that the initial attachment of osteoblast-like cells (MG63) in the hierarchical scaffold was 2.2 times higher than that on the PCL/β-TCP composite scaffold.

Additionally, the proliferation rate of the cells was about two times higher than that of the composite scaffold after 7 days of cell culture. Based on these results, we conclude that the collagen nanofibers and β-TCP particles in the scaffold provide good synergistic effects for cell activity.
Alternative Title
Three-dimensional composite scaffolds consisting of polycaprolactone and β-tricalcium phosphate by using a plasma/rapid-prototyping process
Alternative Author(s)
Yeo, Myung Gu
일반대학원 기계공학과
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Table Of Contents
제 1 장 서론 -------------------------------------- 1
1. 연구의 배경 ----------------------------------- 1
2. 연구의 목적 ----------------------------------- 3

제 2 장 조직공학의 이해 --------------------------- 4
1. 조직공학과 세포담체 --------------------------- 4
2. 합성 고분자 및 생체세라믹스 세포담체 제작 ------- 8
3. 표면개질을 위한 플라즈마 처리 ------------------ 11
4. 참고문헌 -------------------------------------- 12

제 3 장 실험 및 방법 ----------------------------- 33
제 1 절 PCL/β-TCP가 혼합된 세포담체 제작 ------- 33
1. 서론 ------------------------------------------ 33
2. 실험 ------------------------------------------ 36
3. 결과 ------------------------------------------ 40
4. 결론 ------------------------------------------ 45
5. 참고문헌 -------------------------------------- 46

제 2 절 PCL/β-TCP/phloronannin 세포담체 제작 -- 62
1. 서론 ------------------------------------------ 62
2. 실험 ------------------------------------------ 63
3. 결과 ------------------------------------------ 67
4. 결론 ------------------------------------------ 72
5. 참고문헌 -------------------------------------- 73

제 3 절 세포담체의 플라즈마 처리 ----------------- 90
1. 서론 ------------------------------------------ 90
2. 실험 ------------------------------------------ 91
3. 결과 ------------------------------------------ 92
4. 결론 ------------------------------------------ 93
5. 참고문헌 -------------------------------------- 94
조선대학교 대학원
여명구. (2012). 플라즈마/쾌속조형공정을 통한 조골세포 재생용 PCL/β-TCP 세포담체에 대한 연구.
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