유산균 함유 뽕잎분말이 Loperamide로 유도된 흰쥐의 변비에 미치는 영향

Abstract

This study examined the preventive effects of mulberry leaf powder (MLP) containing the lactic acid bacteria (LAB) for loperamide - induced constipation in rats. Animals were divided into nimal and three experimental groups: nomal diet group (N), nomal diet and loperamide treat group(C), 5% MLP and loperamide treat group (ML), and 5% MCP containing LAB and loperamide treat group (MLL). Constipation was induced by subcutaneous injection of loperamide (4.0 mg/kg body weight/day) for the final 5 days of the experiment. Body weight gain was significantry decreased in groups fed ML and MLL compared to the nomal group, even before loperamide was not injected into the rats. After constipation was induced by loperamide injection, food intake was significantry increased in ML and MLL groups compared to the loperamide only group. Numbers of fecal pellet, wet weight offecal pellets and fecal water contents were on significantry increased in ML and MLL group compared C group. However, no difference was seen in the C group compared with the N group. The number of fecal pellets in rat colon and intestinal length were significantry decreased in the loperamide only group. Compared to the nomal group and were significantry increased in ML and MLL treated groups compared to the loperamide only group. Furthermore the transit time and transit speed of the ML and MLL treat groups decreased until it was simiral to the nomal group. These parameters were not significantry different between the ML group and the MLL group. The content of glucose in serum was not significantry different between experimental groups. Serum triglyceride, cholesterol and LDL-cholesterol levels were lowered and HDL-cholesterol level was increased in ML and MLL treated groups. These results indicated that mulberry leaf or mulberry leaf containing lactic acid bacteria could be effective for preventing and threating constipation symptoms.