와편모조류 Heterocapsa circularisquama HcRNAV34 virus like particle의 발현, 정제,자가조립 및 숙주특이적 살조능

Abstract

Virus-like particles (VLPs) have received considerable attention for vaccine, drug delivery, gene therapy and material science applications. Especially, recombinantly expressed VLPs of HcRNAV (a single-standard RNA virus specifically infecting the dinoflagellate, Heterocapsa circularisquama) is considered as a novel potent carrier for harmful algal bloom. In the present study, HcRNAV34 VLPs were expressed in E. coli BL21(DE3) host system and the VLPs were purified using chitin-binding affinity column chromatography. A novel organic synthesized algicidal substance, thiazolidinedione (TD49), was encapsulated into HcRNAV34 VLPs by dissociation and reassociation processes and its encapsulation was detected by HPLC analysis. HcRNAV34 VLPs encapsulating TD49 specifically attached and destroyed the cells of H. circularisquama HU9433-P, which is the host of HcRNAV34 virus, but not the cells of H. circularisquama HY9423, which is the host of HcRNAV109 virus. These results indicate that HcRNAV34 VLPs have considerably high host specificity and is compatible with a common viral property of host specific infection. On the basis of above results, we suggest the possibility which to develop biological control method of harmful algal blooms using HcRNAV34 VLPs embedded with algicidal substances.