TLR2의 활성이 MDPC-23 세포의 부착과 이동에 미치는 영향
- Author(s)
- 신동은
- Issued Date
- 2012
- Abstract
- Odontoblasts and/or dental pulp cells are responsible for tooth repair as well as dentin formation. Adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether Pam3 modulates adhesion and migration of a murine odontoblast-like cell line, MDPC-23 cell and TLR2 signaling is engaged in this process. TLR2 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type Ⅰ collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, JNK, p38, and ERK was examined by Western blot analysis. TLR2 was functionally expressed in MDPC-23 cells. Pam3CSK treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR2 using its antibody restored Pam3CSK-induced adhesion and migration of MDPC-23 cells. These findings indicate that Pam3CSK, an immune activator from gram negative bacteria, can promote adhesion and migration ability of MDPC-23 cells via TLR2.
- Alternative Title
- TLR2 activation promotes adhesion and migration of murine dental papilla derived MDPC-23 cells
- Alternative Author(s)
- Shin, Dong Eun
- Department
- 일반대학원 치의학과
- Advisor
- 안상건
- Awarded Date
- 2013-02
- Table Of Contents
- 목 차 ·············································································· ⅰ
도목차 ·········································································· ⅲ
ABSTRACT ····································································· ⅳ
Ⅰ. 서 론 ········································································· 1
Ⅱ. 재료 및 방법 ·································································· 3
1. 세포 배양 및 시약 ·························································· 3
2. Reverse transcription-polymerase chain reaction (RT-PCR) ······· 3
3. MTT 분석 ·································································· 4
4. Western blot 분석 ··························································· 4
5. 세포 부착 분석 ······························································· 4
6. 세포 이동 분석 ······························································· 5
7. 자료 분석 ····································································· 5
Ⅲ. 결과 ············································································ 6
1. MDPC-23 세포에서 TLR2 발현 및 NF-κB와 MAPK 신호 활성화 ··· 6
2. TLR2 agonist가 MDPC-23 세포 성장에 미치는 영향 ··················· 8
3. TLR2 agonist가 MDPC-23 세포 부착에 미치는 영향 ··················· 9
4. TLR2 agonist가 MDPC-23 세포 이동에 미치는 영향 ·················· 11
Ⅳ. 고찰 ··········································································· 13
V. 참고문헌 ········································································· 15
- Degree
- Master
- Publisher
- 조선대학교 대학원
- Citation
- 신동은. (2012). TLR2의 활성이 MDPC-23 세포의 부착과 이동에 미치는 영향.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/9673
http://chosun.dcollection.net/common/orgView/200000263564
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Appears in Collections:
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- Embargo2012-12-21
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