CHOSUN

TLR2의 활성이 MDPC-23 세포의 부착과 이동에 미치는 영향

Metadata Downloads
Author(s)
신동은
Issued Date
2012
Abstract
Odontoblasts and/or dental pulp cells are responsible for tooth repair as well as dentin formation. Adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether Pam3 modulates adhesion and migration of a murine odontoblast-like cell line, MDPC-23 cell and TLR2 signaling is engaged in this process. TLR2 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type Ⅰ collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, JNK, p38, and ERK was examined by Western blot analysis. TLR2 was functionally expressed in MDPC-23 cells. Pam3CSK treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR2 using its antibody restored Pam3CSK-induced adhesion and migration of MDPC-23 cells. These findings indicate that Pam3CSK, an immune activator from gram negative bacteria, can promote adhesion and migration ability of MDPC-23 cells via TLR2.
Alternative Title
TLR2 activation promotes adhesion and migration of murine dental papilla derived MDPC-23 cells
Alternative Author(s)
Shin, Dong Eun
Department
일반대학원 치의학과
Advisor
안상건
Awarded Date
2013-02
Table Of Contents
목 차 ·············································································· ⅰ
도목차 ·········································································· ⅲ
ABSTRACT ····································································· ⅳ

Ⅰ. 서 론 ········································································· 1

Ⅱ. 재료 및 방법 ·································································· 3
1. 세포 배양 및 시약 ·························································· 3
2. Reverse transcription-polymerase chain reaction (RT-PCR) ······· 3
3. MTT 분석 ·································································· 4
4. Western blot 분석 ··························································· 4
5. 세포 부착 분석 ······························································· 4
6. 세포 이동 분석 ······························································· 5
7. 자료 분석 ····································································· 5

Ⅲ. 결과 ············································································ 6
1. MDPC-23 세포에서 TLR2 발현 및 NF-κB와 MAPK 신호 활성화 ··· 6
2. TLR2 agonist가 MDPC-23 세포 성장에 미치는 영향 ··················· 8
3. TLR2 agonist가 MDPC-23 세포 부착에 미치는 영향 ··················· 9
4. TLR2 agonist가 MDPC-23 세포 이동에 미치는 영향 ·················· 11

Ⅳ. 고찰 ··········································································· 13

V. 참고문헌 ········································································· 15
Degree
Master
Publisher
조선대학교 대학원
Citation
신동은. (2012). TLR2의 활성이 MDPC-23 세포의 부착과 이동에 미치는 영향.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/9673
http://chosun.dcollection.net/common/orgView/200000263564
Appears in Collections:
General Graduate School > 3. Theses(Master)
Authorize & License
  • AuthorizeOpen
  • Embargo2012-12-21
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.