플라즈마 표면 개질한 polycaprolactone scaffold의 골 융합 증진효과

Abstract

Three-dimensional polycaprolactone (3D-PCL) has been widely adopted for biomaterials and biomedical applications due to its slow degradability and good biocompatibility, as well as its good mechanical and thermoplastic characteristics. Surface properties of scaffolds such as hydrophilicity and the presence of functional groups on the surface of scaffolds play a key role in cell adhesion and proliferation. However, PCL has hydrophobic surface and lacks of functional groups, therefore, it is not a good condition on substrate for cell adhesion. PCL surface modification was performed to increase hydrophilic by introducing functional groups. The fabrication of surface containing carboxyl acid groups by plasma has paid considerable attention due to enhancing osteoblast-like cell (MC3T3-E1) attachment and proliferation. Plasma polymerization is a convenient and versatile technique for the fabrication of thin polymer films ranging in thickness from a few nanometers to a few micrometers. In this study, 3D-PCL surface was modified using a low temperature RF discharge plasma with carboxyl group containing monomer such as acrylic acid (AA) and evaluated in vitro its cellular response. The change in wettability and surface morphology of 3D-PCL surface by plasma treatment were determined according to contact angle and field emission scanning electron microscopy. The chemical bonding state and chemical structure of acrylic acid plasma polymerized 3D-PCL surfaces were examined by X-ray photoelectron spectroscopy. The untreated surfaces and AA plasma treated with 3D-PCL surfaces were also examined for their in vitro pre-osteoblast (MC3T3-E1) cells proliferation and differentiation using MTT assay and ALP test. The results of surface characterization showed that thin films of poly acrylic acid was deposited on 3D-PCL surface after AA plasma treating for 15 min under a power of 30, 50, and 70W. These results suggest that carboxyl group containing polymeric thin films on the 3D-PCL surface has an effect on the proliferation and differentiation of MC3T3-E1 cells and to be used potentially in osteo-conductive bone implants.