오염된 자가치아 뼈이식재의 처치방법에 따른 골형성능 비교

Abstract

Purpose The purpose of this study was to evaluate new bone formation and bone to implant contact histomorphometrically using contaminated autogenous teeth bone graft materials which was re-sterilized with EO gas, autoclave and adapted to patients having alveolar bone defect during implant placement procedure.

Method The experiment was conducted using Dentis HAPTITE 3.7mm diameter,10mm length implant and autogenous teeth bone graft aimed at bred 6 hybrid dogs with about 15kg aging 6 to 12 months under same condition. The experiment group was composed to no treatment group, sterilization using EO gas group, and sterilization using autoclave group according to sterilizing method after contamination of autogenous teeth bone graft. Experimented animals were sacrificed after 4 weeks and 8 weeks of post-experiment, clinical evaluation was done during osteocomma collection, and bone to implant contact ratio(BICR) and new bone formation ratio(NBFR) were evaluated using the slides made from collected tissues.

Results The result of bone to implant contact ratio (BICR) of dressing-free group and the sterilization using autoclave group in 4 weeks group showed lower level versus control group, the sterilization using EO gas group, however, it compare to difference with control group. And the result of the dressing-free group in 8 weeks group resulted in lower proportion of implant bone contact, yet the result was not statistically significant. The new bone formation ratio inside bone defect area of conta- minated group and the sterilization using autoclave group in 4 weeks group resulted in lower level versus control group, the sterilization using EO gas group, however, showed no-regardful difference with control group. And in 8 weeks group, the sterilization using autoclave group resulted in significant lower level versus control group, however, other experiment groups showed no-regardful difference.

Conclusion According to the above result, the sterilization using ethylene oxide gas might be considered favorable than high pressure sterilization to the new bone formation and implant osseo-integration in case of re-use of contaminated autogenous teeth bone graft materials.