마이크로 나노 표면구조를 갖는 생체적합성 세포담체 제작과 이에 따른 조골세포의 영향

Abstract

Abstract

The fabrication of micro/nano-surface patterned on the biocompatible scaffolds and analysis of the effects on the cellular activities of osteoblast-like cells(MG63).

Jeon hojun Advisor : Prof. Kim GeunHyung, Ph.D. Department of Mechanical Engineering Graduate School of Chosun University

This thesis present the development of electric-field-aided nano/micro technique (FA-NCT) and its application to investigate the effect of scaffold pattern on the cell behavior in tissue engineering. One of the challenges in tissue engineering is the design of optimal biomedical scaffolds, which can be governed by both physical and chemical properties, such as structural properties (porosity, pore size, and tortuosity) and surfacce characteristics. Of these properties, we observed the effects of nano- to microsized hierarchical surface. To achieve the hierarchical surface structure on poly(3-caprolactone)(PCL) film, we employed an electric-field-aided nano/micro-casting technique (FA-NCT) to mimic a typical natural hierarchical structure, the lotus leaf. The sizes of the microsized structures in lotus leaf replicas were controlled by employing or not employing the electric field during casting. The hierarchical surfaces showed two different roughness ranges for the microsized structure and the nanosized structure: (1) 1.7 0.2 mm and 627 163 nm and (2) 3.2 0.3 mm and 635 127 nm, respectively. To reduce the hydrophobicity in the replicated surfaces, they were coated with phlorotannin (Ph), which is derived from brown algae and has been used as a tissue regenerating material due to its hydrophilicity and various growth factors. Osteoblast-like-cells (MG63) were cultured on various surfaces. The hierarchical structure induced high cell viability compared to that of the smooth surface. Moreover, calcium deposition was enhanced when the roughness of the microsized structures in the hierarchical structure was increased. From the results, we suggest that control of the formation of micro-structure in a hierarchical structure is an important design factor along with the optimal nanostructure.