Three New Compounds from Panax ginseng increase Interleukin-12 Production in LPS-Activated Macrophages
- Issued Date
- 2012
- Abstract
- 식물에서 바이러스 감염 및 암을 포함하여 세포면역의 증가를 필요로 하는 질병의 치료에 초점을 맞춘 지속적인 연구의 일환으로서 인삼의 잎으로부터 3개의 신규 화합물과 2개의 기지 화합물을 분리하였다. 신규 화합물의 구조는 스펙트럼 분석법에 의하여 3,12-dione-6α,20-dihydroxy-dammar-24-ene (1), 27-demethyl-dammar-20(22)-E,24-dien-3β,6α-diol-25-one (2), and 12β,23-epoxy-3β-hydroxydammar-24-ene-20-O-[β-D-glucopyranoside] (3) 로 밝혔다. 분리된 화합물 1 - 5를 면역 조절 활성에 대한 검사 결과 화합물 2, 3 그리고 20(S)-Rg2 는 LPS가 활성화된 마우스의 복막대식세포에서 Inteleukin-12의 생산성을 증가시켰다. 또한, 화합물 2는 ConA가 활성화된 비장세포에서 Th2 반응을 중재시키는 cytokines, IL-4, 그리고 IL-6 을 감소시킨다. 이러한 결과는 화합물2가 Th1반응을 강하게 유도하고 더 나은 결과로 바이러스와 암에 대하여 보호 역활을 할 수 있음을 제안한다.
- Alternative Title
- 인삼으로부터 LPS-자극된 마크로파지에서 IL-12분비를증가시키는 진세노사이드 분리 및 정제
- Alternative Author(s)
- Tran Tien Lam
- Affiliation
- 조선대학교
- Department
- 일반대학원 약학과
- Advisor
- 오원근
- Awarded Date
- 2012-08
- Table Of Contents
- List of Tables ············································································································································· iii
List of Figures ············································································································································ iv
ABSTRACT ····················································································································································· x
1. Introduction .................................................................................................................................. 1
1.1 Panax ginseng C. A. Meyer (Araliaceae) .................................................................................1
1.2 Cytokines .................................................................................................................................2
2. Materials and Methods .................................................................................................................. 4
2.1 Materials ..................................................................................................................................4
2.1-1. Plant material .................................................................................................................... 4
2.1-2. General experimental procedures ..................................................................................... 4
2.2 Methods ...................................................................................................................................5
2.2-1. Extraction and isolation .................................................................................................... 5
2.2-2. Isolation of murine peritoneal macrophages and ELISA assay. ........................................ 9
2.2-3. Preparation of spleen lymphocytes and measurement of cytokine levels. ........................ 9
3. Results and Discussion .................................................................................................................11
3.1 Structure determination of ginsenosides from Panax ginseng ................................................ 11
3.1.1 Chemical structure of new compound 1........................................................................... 11
3.1.2 Chemical structure of new compound 2........................................................................... 16
3.1.3 Chemical structure of new compound 3........................................................................... 24
ii
3.1.4 Chemical structures of known compounds ...................................................................... 30
3.2. Isolated compounds increase IL-12 production on LPS-activated macrophages ...................34
3.3. Inhibition of NO production on LPS-activated macrophages ................................................39
3.4. Compound 2 inhibited strongly pro-inflammatory cytokines on Con A-activated splenocytes.
.....................................................................................................................................................40
4. References .................................................................................................................................. 46
5. ACKNOWLEDGEMENTS……………………………………………………….…………..45
- Degree
- Master
- Publisher
- 조선대학교
- Citation
- 뜨란띠엔람. (2012). Three New Compounds from Panax ginseng increase Interleukin-12 Production in LPS-Activated Macrophages.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/9532
http://chosun.dcollection.net/common/orgView/200000263330
- Appears in Collections:
- General Graduate School > 3. Theses(Master)
- Files in This Item:
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