초음파 조사시간에 따른 혈소판풍부섬유소내 성장인자의 유리변화

Abstract

Low intensity pulsed ultrasound (LIPUS) and platelet-rich fibrin (PRF) have recently been used as a means of enhancing wound healing and new bone formation in regenerative therapy. The aim of this study was to evaluate the release of three growth factors, platelet-derived growth factors-BB (PDGF-BB), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) from the PRF clot exudate serum irradiated with LIPUS. Blood collection was carried out on 10 healthy volunteers (non-smoker males from 22 to 27 years of age). Blood of 20 ㎖ was collected from each volunteer. Blood samples were taken in 10 ㎖ glass-coated plastic tubes (without anticoagulant) that were immediately centrifuged at 3,000 rpm (approximately 400g) for 10 minutes. LIPUS (240mW, 15-minute) irradiation was applied to the PRF membranes during 0-, 15-, 30-, 45- and 60-minutes, respectively. The values of PDGF-BB, IGF-1, and VEGF from these samples after stimulating with LIPUS was quantified by ELISA. All experiments were triplicated. The values obtained were analysed statistically the difference of the each average. The experimental results were tested by using paired t-test, with a 5% significance threshold. Within the limits of this study, the results showed that LIPUS irradiation influences the release of PDGF and VEGF from PRF as its irradiation time goes.