천일염으로 제조한 김치와 된장의 암세포 성장억제 효과

Abstract

To investigate the growth inhibitory effect of Kimchi and Doenjang prepared with various solar salt, water extracts and methanol extracts the Kimchi and Doenjang were measured on AGS human gastric adenocarcinoma cells, HT-29 colon carcinoma cells and BJ human foreskin normal cells using MTT assay. Kimchi was prepared, chinese cabbages were brined with addition of purified salt, four years aged solar salt, and topan solar salt and mixed with other ingredients. The final salt concentration was adjusted into 2.2～2.4% (w/v) with each salt and the Kimchi was fermented at 7℃. When acidity of the Kimchi reached around 0.5～0.6%. The water extracts of all Kimchi samples showed growth-inhibitory effect on cancer cells, however the difference among the used salts did not observed. The methanol extracts of all Kimchi samples showed higher growth-inhibitory effect than the water extracts. The methanol extract of four years aged solar salt Kimchi(AGS: 73%, HT-29: 48%) showed higher growth-inhibitory effect than the purified salt Kimchi(AGS: 52%, HT-29: 39%). In addition, morphological changes of cancer cells(AGS, HT-29) and decreased their cell numbers were observed, when the methanol extract of four years aged solar salt Kimchi was treated into AGS and HT-29 cells. However all Kimchi extracts little cell toxicity for BJ normal cells. Doenjang prepared with various kinds of solar salt. Doenjang was prepared using the bacterial koji and six kinds of salt(purified salt, one year aged solar salt, four years aged solar salt, topan solar salt, boiled solar salt and gojil solar salt) with 12%(w/w) of NaCl concentration. The Doenjangs were fermented and aged for 18 months. The water and methanol extracts of the Doenjang samples showed growth-inhibitory effects on cancer cells. The methanol extracts of Doenjang prepared with four-years aged solar salt(AGS: 55%, HT-29: 48%) stronger growth-inhibitory effect than Doenjang with purified salt(AGS: 42%, HT-29: 38%). In addition, decreased cancer cell numbers and morphological changes of cancer cells were observed when methanol extract of Doenjang prepared wIth four years aged solar salt was treated to cancer cells(AGS, HT-29). However, all extracts of Doenjangs did not showed any growth inhibitory effect on BJ normal cells.