생체모사형 하이브리드 세포담체 제작에 대한 연구

Abstract

Tissue engineering is a new and exciting technique, which has the potential to create tissues and organs. This technology involves the in vitro seeding and attachment of human cells onto a scaffold. In this technique, the scaffold, which is a substitute to grow the injected cells or the environmental cells in human body, has been one of prominent factors in tissue engineering. To acquire successful tissue regeneration, the scaffold should have several requirements, such as porous structure, biodegradable, biocompatible properties, and proper mechanical properties: Of these requirements, highly porous and interconnected pore structure are the most important factors, which can accommodate injected cells and exchange nutrients between scaffolds and environment condition. There are so many methods, freeze-drying, salt-leaching, solid freeform fabrications(fuse deposition modeling, 3D printing, 3D plotting, and sterolithography etc.), to fabricate various porous scaffolds. Of these methods, freeze-drying can fabricate highly porous scaffolds, which has high surface-to-volume ratio and similar physical structure like the extracellular matrix (ECM). However, although freeze-dring method provide an optimal biological structure and easy process-ability, low mechanical property is a main drawback. To overcome this problem, we propose a new and simple hybrid fabrication process supplemented with rapid-prototyped method and simple coating method. Firstly, rapid-prototyped poly(e-caprolactone) scaffolds were fabricated with multi-layered structure. Secondly, in the scaffolds collagen/b-TCP solution was injected to coat the surface of PCL struts. Finally, the scaffolds were freeze-dried to obtain internally designed collagen-structure. Fabricated hybrid scaffolds were characterized with various physical properties, including water contact angle, water absorption ability, and mechanical properties, and cellular activities. From these works, we believe that the hybrid scaffolds can be one of potential biomedical materials for bone tissue regeneration.