바이오 디젤 생산을 위한 해양 미세조류(Dunaliella tertiolecta,Tetraselmis chui, Nannochloropsis oculata)최적배양에 관한 연구

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Recently, there is a growing interest in microalgae and the use of microalgae focused on the production of various high valued metabolites used for food, pharmaceuticals, and cosmetics. The rate of depletion of fossil fuels and the effect of greenhouse gas emissions on global climate change evoke much interest in biodiesel.
Microalgae have been issued much more as the factory for biodiesel production due to their several potential advantages ; high yield of neutral lipid/oil as 20-50%, high growth rate, viability in extremely arid condition, doing major roles in wastewater treatment, removers of carbon dioxide and producers of valued chemicals and much higher biomass production in a suitable vessel compared to those of terrestrial plants in the same area.
In this study, three species of marine microalga Tetraselmis chui, Dunaliella tertiolecta and Nannochloropsis oculata were cultured to investigate three important growth factors such as CO2 concentration, light intensity and light-cycle. The cell growth rate, biomass productivity, oil content and fatty acid composition of T. chui, D. tertiolecta and N. oculata, candidates for biodiesel production were measured using 1L photobioreactor. The productivity of cell mass and oilcontent increased with repeated fed-batch culture.
The optimal conditions for cell growth and oil production at 23℃, light intensity 110μmol/m2/s, and light cycle 14:10(L:D), it was transferred to 1L photobioreactor its medium composition was F/2-Si × 3 medium. And filtered air containing 2% CO2 was injected into the conical bottom part of the photobioreactor at a constant flow rate of 1vvm.
The repeated fed-batch system was adopted to increase the cell mass and oil content productivity for 2 weeks. The cell was harvested every other days. Biomass productivity and oil content of T. chui were 0.415 g/L/day and 0.170 g/L, respectively. Those of D. tertiolecta were 0.478 g/L/day and 0.173 g/L, respectively.
Alternative Title
Optimization of microalgae(Dunaliella tertiolecta,Tetraselmis chui, Nannochloropsis oculata) culture conditions for biodiesel production.
Alternative Author(s)
Kim bo ram
일반대학원 화학공학과
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Table Of Contents
List of Figures iv
List of Tables vi

제 1장 서론 1

제 2장 이론 3
1. 미세조류 3
2. 미세조류 성장에 영향을 미치는 요소 4
2.1 광원 5
2.2 온도 7
2.3 영양분 공급 7
2.4 CO2 공급 8
3. 바이오 디젤 9

제 3 장 실험 재료 및 방법 10
1. 미세조류 종류 및 배양방법 10
1.1 미세조류 종류 및 배지 10
2. 배양조건 12
2.1 전 배양 12
2.2 광 생물반응기 13
2.3 광 생물반응기 운전조건 15
3. 분석방법 16
3.1 조도 측정 16
3.2 미세조류의 농도측정 16
3.3 질산원과 인산원 농도 최적화 16
3.4 FAMEs(fatty acid methyl ester) 측정 17
3.5 GC(gas chromatography) 분석 방법 17

제 4 장 실험결과 18
1. 우수종 미세조류 선별 18
1.1 성장율이 우수한 미세조류 선별 18
2. 광 조건에 따른 미세조류 성장 20
2.1 조도 20
2.2 빛 주기 22
2. CO2 농도의 영향 24
2.1 Tetraselmis chui 24
2.2 Dunaliella tertionlecta 27
2.3 Nannochloropsis oculata 30
3. 인산염 농도의 영향 33
3.1 Tetraselmis chui 33
3.2 Dunaliella tertiolecta 36
3.3 Nannochloropsis oculata 39
4. 질소원 농도의 영향 42
4.1 Tetraselmis chui 42
4.2 Dunaliella tertiolecta 45
4.3 Nannochloropsis oculata 48
5. 질소원과 인산원 최적농도 51
6. 반복유가식 배양공정 개발 56
6.1 Tetraselmis chui 56
6.2 Dunaliella tertiolecta 59
6.3 Nannochloropsis oculata 62
7. 비닐백을 이용한 배양공정 65
7.1 Tetraselmis chui 65
7.2 Dunaliella tertiolecta 68

제 5 장 결론 71

참고문헌 73
김보람. (2011). 바이오 디젤 생산을 위한 해양 미세조류(Dunaliella tertiolecta,Tetraselmis chui, Nannochloropsis oculata)최적배양에 관한 연구.
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