미세조류 배양을 위한 평판형 광생물반응기에서의 성장조건 최적화에 관한 연구

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n recent years, the demands of bioenergy utilization are increased as the importance of environmental conservation become bigger. At first, the mass cultivation and high concentration of microalgae using photobioreactor are required in order to convert bio resources to applicable energy source. The open-pond system were developed to improve the microalgae cultivation and massively operated for the product derived from a lot of micro-organisms. However, in the aspect of the efficiency of light utilization, the closed photobioreactor system is far superior than open-pond. The recently developed flat-panel photobioreactor can efficiently cultivate microalgae in the small space. For the reason many researchers are focusing on the development of this system.
In this study, the effect of the structure and the size of photobioreactor is investigated in order to obtain the optimal culture conditions of microalgae and scale-up of the reactor. Chlorella is selected based on the lipid-rich and easy-to-cultured. The flat-panel photobioreactor with the various size, which are 5 L, 15 L, 25 L, and 50 L are fabricated and two types of 15 L photobioreactor with the different structure are constructed.
Chlorella was cultivated in both the photobioreactor with blade and without blade to investigate the effect of structure. The experimental result shows that the bioreactor with blade showed rapider growth rate of Chlorella than the reactor without blade. This result means that the light source could be effectively provided to Chlorella in the photobioreactor with blade because of the active circulation of CO2 and medium, and non(or minimum)-precipitation. Therefore, the airlift system and the static stirring system should be applied to photobioreactor for the high condensed cultivation.
The experiment which investigates the relationship between the size of photobioreactor and the grows rate of Chlorella is performed to verify the feasibility of scale-up. The flat-panel photobioreactors with 5 L, 25 L, 50 L capacity and the same structure are used. According to the result, it is verified that the smaller photobioreactor, the rapid growth rate was shown since the higher density of Chlorella makes the light penetration ratio smaller. However the difference of growth rate was insignificant. Therefore, it is expected that the bigger size of photobioreactor can be more efficient in the view of mass production.
Alternative Title
A Study on optimization of the growth conditions for microalgae cultivation in the flat panel photobioreactor
Alternative Author(s)
Oh, Ju-Yeol
조선대학교 일반대학원 기계공학과
일반대학원 기계공학과
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Table Of Contents
목 차


제 1 장 서 론 1
제 1 절 연구 배경 1
제 2 절 연구 동향 4
1. 광생물반응기의 개발 동향 4
제 3 절 연구 내용 7

제 2 장 미세조류 8
제 1 절 미세조류의 일반적 특성 8
제 2 절 Chlorella sp. 11

제 3 장 평판형 광생물반응기 12
제 1 절 평판형 광생물반응기 제작 12
제 2 절 평판형 광생물반응기에서의 배양시스템 16

제 4 장 평판형 광생물반응기에서의 미세조류 배양 21
제 1 절 실험 장치 구성 21
1. 미세조류 선정 21
2. 시스템 구성 23
제 2 절 분석 방법 24
제 3 절 Chlorella sp. 배양 25
1. 배지조성 25
2. 조건 변화에 따른 배양 28
가. 광생물반응기의 구조 변화에 따른 배양 28
나. 광생물반응기의 용량 변화에 따른 배양 34

제 5 장 결 론 42
1. 연구결과 42
2. 향후 연구방향 43

참 고 문 헌 44
조선대학교 일반대학원
오주열. (2011). 미세조류 배양을 위한 평판형 광생물반응기에서의 성장조건 최적화에 관한 연구.
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General Graduate School > 3. Theses(Master)
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