구강소독제의 적용 시간에 따른 타액 내 세균수의 감소 효과 비교

Abstract

Oral sterilization with the oral antiseptic agent during oral and maxillofacial surgery is a significant factor in terms of preventing the postoperative infection. For this reason, various kinds of oral antiseptic agents are used to reduce intraoral bacteria. The purposes of this study are to establish the effective intraoral application time of antiseptic agent and the utility of mechanical irrigation. Povidone-iodine (PVI) and Chlorhexidine gluconate (CHX) was used, and colony-forming unit (CFU) was calculated for the salivary bacterial counts. Total 80 subjects were selected for this study. Saliva secreted at resting state was taken. Experimental groups kept 10% PVI and 0.2% CHX for 20 and 40 seconds and irrigated with sterilized normal saline respectively according to experimental protocol. Then the saliva was taken and diluted with phosphate buffered saline and then plated onto 5% sheep blood agar plates which were incubated. Formed CFU after incubation was counted. After application of PVI and CHX, every experimental groups showed statistically significant decrease of CFU (P<0.01). The group 2 (PVI, 40seconds) showed more significant reduction rate of CFU than group 4 (CHX, 40s) (P<0.01). The group 6 (PVI, 40s, irrigated) showed more significant reduction rate of CFU than group 2 (PVI, 40s) (P<0.01). The group 2 (PVI, 40s) showed more significant reduction rate of CFU than group 1 (PVI, 20s) (P<0.01). In summary, application of PVI for 40 seconds and then irrigation with sterilized normal saline showed the best result. Moreover, the better results came with antiseptic, with mechanical irrigation, as times go by, PVI than CHX.

Keywords: povidone-iodine, chlorhexidine, mechanical irrigation, colony forming unit