Streptomyces sp. CS326 균주가 생산하는 지질분해효소의 정제 및 특성 분석.

Abstract

In an attempt to isolate a biocatalyst able to catalyze biodiesel production from microbial source, Streptomyces sp. CS326 was screened from hundreds of soil isolates collected from various parts of Korea. In 16S rRNA sequence analysis, the strain showed high degree of similarity with Streptomyces xanthocidicus(99.79%), therefore it is classified as Streptomyces sp. CS326. An extracellular lipase produced by the strain(LP326) was purified using a single step gel permeation chromatography on Sepharose CL-6B. Molecular weight of LP326 was estimated to be 17kDa by SDS-PAGE. The activity was optimum at 40°C and pH7.0, and was stable at pH5.0-8.0 and below 50°C. It preferred p-nitrophenyl palmitate(C16), along chain substrate; and Km and Vmax for the substrate were determined to be 0.24mM and 4.6mM/min mg, respectively. First N-terminal amino acid sequences were APDLVALQSE, which are different from so far reported lipases. LP326 catalyzed biodiesel production using methanol and various oils; therefore, the enzyme can be applicable in the field of bio-fuel.