티타늄표면에 SLA처리와 알칼리 처리에 따른 조골모세포의 증식 및 분화에 관한 연구

Abstract

Titanium (Ti) have been widely used as biomaterials due to electrical and thermal conductivity and mechanical properties. To improve the biocompatibility, several surface treatment methods were suggested. Micro- and nano topography of Ti implant surface acts on major factor for osteoblast cell adhesion, proliferation and differentiation. In this study, we prepared nano- and micro structured TiO2 surface and evaluated the biological activity of osteoblast like cell on the modified TiO2 surface. TiO2 surfaces of micro- and nano-mesh structured were prepared by SLA and alkaline treatment. Surface morphology and composition were characterized by Field Emission scanning electron micrographs (FE-SEM), and X-ray diffraction (XRD). To evaluate the MC3T3-E1 cell proliferation and differentiation on modified TiO2 surface, MTT assay and ALP activity were used. Alkali treated Ti surface showed nano-mesh TiO2 topographic characteristics. As the increase of immersing time in alkali solution, nano-mesh size was gradually increased. SLA treated Ti surface showed good proliferation and differentiation of MC3T3-E1 cell compared to that of nano-mesh structured Ti surface.