산수유 열매 추출물 분리 및 생리활성 연구

Abstract

Cornus officinalis (C. officinalis) Sieb. et Zucc. Cornaceae, which is commonly called Japanes cornel, is widely distributed in China and Korea. The fruit without the seed, is called sanshuyu and has been used for at least 2,000 years in Chinese herbal medicine. The total iridoid glycoside content of C. officinalis, including loganin, morroniside and other minor constituents, possesses a number of pharmacological activities such as mitigating the vascular complications of diabetes, depressing immunity, rheumatoid arthritis, cerebral ischemiar eperfusion injury through multiple action mechanisms, and against dia betic metabolism. It is used principally to reduce unusually active secretions, including copious sweating and excessive urine. It is known that C. officinalis hot water extract has an antibacterial action, anti-histamaine, and anti-acetylc holine activity in the small intestine of the guinea pig.

This study was carried out to investigate biological activities of C. officinalis fruits. Fruit extracts were prepared by ethanol extraction method and then fractionated with hexane, ethylacetate (EtOAc), butanol (BuOH) extract to get active compounds. The antioxidant activities of extracts were assayed by total polyphenolics content, DPPH radical scavenging, superoxide dismutase (SOD) -like, and catalase (CAT) activities. The DPPH radical scavenging activity of the fruits extracts showed a considerable RC50 value, 234.51 (ethanol extract), 140.60 (hexane extract), 25.60 (EtOAc extract), 110,0 (BuOHextract), 500,00 (wat er extract) ㎍/㎖, respectively. Ethylacetate extract showed higher activity tha n that of BHT standard antioxidant (RC50 101.00 ㎍/㎖). The total SOD-like enzym e activity was found to be 17.80 U/mg and CAT activity was 13.90 U/mg and the co ntents of total polyphenolics was 27.17 mg/kg. Moreover, five compounds were is olated from EtOAc fraction of Cornus officinalis fruits and them were identified with galic acid, cornuside, (7S)-O-methyl morroniside, and (7R)-O-methylmorronis ide, loganin, which have antioxidant activity. These results suggested that C. o fficinalis fruits would be an alternative antioxidant source, based on natural p lant resources.