효소처리를 이용한 해조류의 셀룰로오스 계열 당화공정 개발

Abstract

In this paper, the monosaccarification process from various seaweeds was developed. Sea alagae consist mainly of 40 ~ 70 % polysaccharides, 10 ~ 20 % proteins, and residual low molecular weight compounds such fatty acid, free amino acid and amine. This process just used immobilized enzymes to produce the simple sugars from the seaweeds. The magnetically separable polyaniline nanofiber was used as a carrier of the enzymes. The immobilized enzymes were developed for the recycle of enzyme and enhanced enzyme stability. The residual activity of immobilized enzyme was over 50 % after 15 days incubation at 55°C. Glucose was produced from various polysaccharides, Laminaria japonica, Capsosiphon fulvescens and Gelidium amansii with magnetically separable immobilized enzyme. Simple sugar production rate with the seaweeds were 20 mg / 1 g and showed high decomposition rate due to high mass transfer. After 10 times recycle, the residual activity of immobilized enzyme was over 70 %.