초임계 이산화탄소를 이용한 참당귀 추출물의 항산화 및 미백효과에 대한 연구

Abstract

The aim of the study was to screen the bioactive substance from Angelicagigas Nakai for cosmetic applications. We developed an optimized method of supercritical carbon dioxide for the extraction of decursin and decursinol angelate from Angelica gigas Nakai. Further, we evaluated the antioxidant activity and whitening effect of the extracts and the extracts also subjected for the stability and patch tests. According to the HPLC analysis the result revealed that the contents of decursin and decursinol angelate was 200 bar and 60°C respectively. In the case of antioxidant and whitening tests we have used water extracts, ethanol extracts and commercial products for the comparison. The results showed that the free radical scavenging activity were 95.5% for supercritical extract, 93.32% for water extract 90.71% for organic solvent extract and 92.2% for BHT(Butylated Hydroxy Toluene). The tyrosinase inhibitory activities and DOPA-oxidase inhibitory activities were found to be 58.82-52.85% for supercritical extract, 6.32-15.33% for organic solvent extracts, 35.73-30.70% for organic solvent extracts and 58.70-55.61% for arbutin respectively. The data reported that supercritical extract had the higher inhibitory effect than other extracts. The stability was maintained for 60 days the pH was 6.3-6.9 and no changes in color and odor was observed. Therefore, the Angelica gigas Nakai supercritical carbon dioxide extract have remarkable antioxidant and whitening effect and thus we propose that it can be effectively used for the development of functional cosmetics.