작약 추출물의 아토피 피부염 효과

Abstract

Atopic dermatitis is an intensely pruritic, chronically relapsing inflammatory skin disease. Recent studies have shed light on how the complex interrelation of genetic, enviromental, immunologic, and pharmacologic factors contributes to the development of Atopic dermatitis. The clinical phenotype of Atopic dermatitis varies with age and may differ during the course of disease58. The eczematous lesions may present with acute (oozing, crusted, eroded vesicles or papules on erythematous plaques), subacute (thick and excoriated plaques), and chronic (lichenified, slightly pigmented, excoriated plaques) forms. Current methods of treatment include atopic dermatitis, humectant, and treatment with corticosteroids is common to. However, long-term side effects of steroid use indicate that. Efficacy in atopic dermatitis, many researchers are interested about the natural and the use of herbal medicines in the therapeutic treatment of atopic dermatitis has been suggested recently. In this study, the effect of the extract from Paeonia lactiflora root on atopic dermatitis was evaluated by determining their effects on antimicrobial activity, DPPH radical scavenging activity, polyphenol's content, flavonid content, nitric oxide production, and β-hexosaminidase release. Microbial strains and animal cell lines used the experiments were S.epidermidis, S.aureus, C.albicans and RAW 264.7 cell, RBL-2H3 mast cell. The extracts of Paeonia lactiflora root was prepared by ethanol extraction. Their Antimicrobial activity against S. epidermidis, S. aureus, C. albicans examined by the paper disc agar diffusion assay. Inhibition zone of the ethanol extract(100㎎/㎖) against S.epidermidis, S.aureus, C.albicans were 6.3, 5.0, 5.6 mm, respectively. The antioxidant activities of extracts was used the method of DPPH radical and nitrite scavenging assay, total polyphenolics and flavonoid contents. The DPPH radical scavenging activity of the extract assayed at various concentrations (15.5 ~ 500㎍/㎖). 500㎍/㎖ concentration showed hight activity more than 90%, similar activity to BHT and scavenging activity increased in a dose-dependent manner. In this study, we evaluated the total phenolic content and flavonoid content of ethanolic extract from Paeonia lactiflora root. The extract contained 29± 3㎍/㎖ of total phenolics and 12.7± 3.1㎍/㎖ of flavonoids. Total phenolics content was higher than flavonoids. The in vitro anti-inflammatory activity of the extract in lipopolysaccharide(LPS)-induced RAW.264.7 cells were investigated. The extract inhibited nitric oxide production in LPS-stimulated RAW 264.7 cells. Furthermore, we examined cell viability of RBL-2H3 mast cell and β-hexosaminidase assay for detecting anti-histamic effect in the extract. At the concentration of 10, 25, 50, 100, 250㎍/㎖ of the ethanol extract, β-hexosaminidase release were inhibited 12.03, 16.91, 53.74, 58.70, and 80.24%, respectively. These results suggest that the root extract of Paeonia lactiflora could be provide a considerable plant resource for the treatment of atopic dermatitis.