울금(Curcuma longa L.)로부터 신종 인플루엔자 A (H1N1) 뉴라미니다제 저해제인 커큐미노이드

Abstract

The emergence of drug-resistant influenza viruses and the threat of pandemics highlight the need for new and effective antiviral agents. As part of an ongoing anti-influenza screening program of natural products, three new (1?3) and ten known (4−13) curcuminoids were isolated from a methanol extract of Curcuma longa L. All compounds had strong inhibitory effects on the neuraminidases from two influenza viral strains, H1N1 and H9N2, as noncompetitive inhibitors with IC50 values ranging from 6.18 to 40.17?g/mL and 3.77 to 31.82 ?g/mL, respectively. Furthermore, they exhibited significant inhibitory activity against the neuraminidases from novel influenza H1N1 (WT) and oseltamivir-resistant novel H1N1 (H274Y mutant) expressed in 293T cells. In addition, the detailed relationships of structure and activity by the compounds were investigated. These results suggest that the curcuminoids from C. longa may be potential supplemental molecules in the battle against influenza A (H1N1).