새송이 버섯에서 재조합 인간 인터루킨-32 생산

Abstract

Interleukin-32 (IL-32) is a multifunction cytokine produced mainly by T-cell, natural killer cells and epithelial cells after stimulation with IL-2,IL-12 plus IL-18, and IFNγ, respectively. IL-32 exhibits properties of proinflammatory cytokines, including the induction of inflammatory cytokines such as IL-1β, IL-6, IL-8, TNFα and chemokines, and enhances morphological alterations of the human monocyte cells into macrophage-like cell. Recombinant interleukin-32 can be produced and found to be useful in many fields. Until now, there is no report on recombinant hIL-32 expressed in basidomycete fungus, Pleurotus eryngii. In this study, we examined whether the popular edible mushroom Pleurotus eryngii could support the expression of novel protein hIL-32. A binary vector pCAMBIA-1304 containing the hIL-32 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens mediated transformation. The expression of hIL-32 was confirmed by PCR, Southern blot and Western blot analysis. These results suggest that pleurotus eryngii expression system can be an effective for the production of rhIL-32 at economically relevant level.