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Abstract

ABSTRACT

Effects of Barley Leaf Powder on Lipid Metabolism in Rats and its Antidiabetic Activity

Lee, Yu-Mi Advisor : Prof. Lee, Myung-Yul, Ph. D. Department of Food and Nutrition, Graduate School of Chosun University

Barley (Hordeum vulgare L.) is a plant widely distributed and cultivated with food goals, thus, its economical importance is very marked. By these reasons, it has been studied from different point of views. The barley leaf known to contain strong antioxidants such as superoxide dismutase (SOD), vitamin C, vitamin E and β-carotene. Also, recent study has confirmed that barley leaf extract has antioxidant activity, free-radical scavenging activity, reducing power, and superoxide dismutase-like activity. Therefore the objective of this study was conducted to investigate the effects of powdered barley leaf on major chemical components analysis, antioxidant activities, lipid metabolism, and antidiabetic activities. This study was investigated on the antioxidative activity of 80% ethanol extracts from young barley leaf. Total polyphenol, flavonoid and DPPH radical scavenging activity contents of barley leaf ethanol extract were highly. Also, rancimat test was highly and it was similar to the BHT. The results of effects of barley leaf powder on lipid metabolism in rats fed high fat diet were as follows; The body weight gain and Food intake were gradually decreased in the barley leaf powder fed groups compared with the HF group. The serum AST, and ALP activities were significantly decreased by barley leaf powder administration. Levels of serum HDL-cholesterol level decreased in the HF group and markedly increased in the barley leaf powder fed groups. Levels of total cholesterol and triglyceride in liver and adipose tissues were lower in barley leaf powder administered groups than in HF group. The activities of heparin-releasable lipoprotein lipase (HR-LPL) and total-extractable LPL (TE-LPL) in adipose tissues were increased in HF group than that of ND group, but those of barley leaf powder administered groups were significantly decreased. This study was to investigate the effect of barley leaf powder of streptozotocin(STZ)-induced diabetic rats. The blood glucose of STZ group was increased than that of N group, and those of STZ-BH group was significantly decreased. Serum insulin concentrations were decreased with STZ group, however, the increased after barley leaf powder administration. The concentration of fructosamine was significantly decreased by barley leaf powder groups. This study was conducted to investigate the effects of powdered barley leaf on antidiabetic activities in an animal model of type 2 diabetes mellitus, db/db mice for 6 weeks. The serum blood glucose and serum insulin of Diabetes group was increased than that of Normal group, and those of barley leaf powder administered groups were significantly decreased. The serum C-peptide of barley leaf powder administered groups were significantly increased between Diabetes group. The contents of total glycated hemoglobin was significantly decreased by barley leaf powder groups. The concentration of Albumin and total protein were significantly increased by barley leaf powder groups. The disaccharidse activities, such as maltase, sucrase and lactase in small intestine were inhibited by barley leaf powder groups. These results suggest that barley leaf powder may improve lipid metabolism of serum, liver and adipose tissue and potentially reduce lipid storage. Also, the barley leaf powder may improve antioxidant activities, lipid metabolism of serum, liver, adipose tissue and potentially reduce lipid storage. And the barley leaf powder might be effective on diabetic mellitus.