모과나무(Chaenomeles sinensis Koehne.)의 화학 성분과 IL(interleukin)-6의 저해 효과

Abstract

Chaenomeles sinensis Koehne (Rosaceae) is an deciduous tree that is found throughout China, Korea and Japan. In previous studies on the fruits of C. sinensis, some triterpenoid compouds such as oleanolic acid, tormentic acid were reported. We searched for the phytochemical constituents of the methylene chloride and n-butanol soluble fraction of the branches of C. sinensis. β-sitosterol (1), betulin (2), 1-β-D-glucopyranosyloxy-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L- rhamnopyranoside (4) were isolated by the repeated use of silica gel, PTLC RP-18 and RP-18 column chromatography. The chemical structures of compounds 1-4 were determined by the basis of physicochemical properties and spectroscopic methods such as 1D and 2D NMR. For the isolated compounds, the inhibitory activity of IL-6 production in TNF-α stimulated MG-63 was examined. Among the isolated compounds, betulin (2), 1-β-D-glucopyranosyloxy-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L- rhamnopyranoside (4) showed inhibitory effects on IL-6 production in TNF-α stimulated MG-63.