백출(Atractylodes japonica)의 추출물이 인체 유방암 MDAMB231 세포주에 미치는 영향

Abstract

백출(atractylodes japonica) extract로써 유방암의 억제증식효과를 실험한 결과 메탄올 추출에서 시간과 농도의존성에서 유익한 효과는 보이지 않았으나 열수 추출에서는 48시간의존성에서 고분자 900에서 35%억제효과를 보였다.

백출(atractylodes japonica) COX-2와 iNOS로써 유방암의 억제증식효과를 실험한 결과 메탄올 추출에서 암세포증식억제와 암표적인자의 발현억제기능이 COX2(60%), iNOS(99%)의 효과를 보였다.

열수 추출에서는 암세포증식과 암표적인자의 발현억제기능이 COX2(60%), iNOS 300에서 다소 억제효과를 보였다.

이상과 같은 점으로 보아 백출의 메탄올과 열수추출물이 식품가공을 통해서 식품이 될 수 있는 가능성은 보여주었다고 할 수 있으며 유방암의 COX-2와 iNOS의 억제증식 효과가 다소 보임으로 isolation과 purification test를 거쳐 좀더 다양하고 정밀하게 검토된다면 임상적 효능을 가져올 수 있을 것으로 기대된다.|Objective : This study is on the effects of the anti-cancer activity of Atractylodes japonica extract in MDAMB231 invasive human breast cancer cell lines.

Methods : MTS assay was used to detect the effects of Atractylodes japonica extracts on the MDAMB231 invasive human breast cancer cell lines viability. And Western Blotting was used to see the effects of Atractylodes japonica extracts on cyclooxygenase-2 (COX-2), iNOS expression.

Results : Methanol extracts of Atractylodes japonica reduced the MDAMB231 invasive human breast cancer cell lines depending on time and concentration. Also it reduced cyclooxygenase-2 (COX-2), iNOS irrespective of concentration.

MTS assay results, 1. Methanol extracts of Atractylodes japonica reduced the MDAMB231 invasive human breast cancer cell lines depending on time. 2. Methanol extracts of Atractylodes japonica reduced the MDAMB231 invasive human breast cancer cell lines depending concentration. Cell survival rate decrease depending on time and concentration especially, it reduced significantly at 900 μg/ml.

3. Hot water extracts of Atractylodes japonica reduced the MDAMB231 invasive human breast cancer cell lines depending on time. 4. Hot water extracts of Atractylodes japonica reduced the MDAMB231 invasive human breast cancer cell lines depending concentration. Cell survival rate decrease depending on time and concentration especially, it reduced significantly at 900 μg/ml.

Western blot results, 5. Methanol extracts of Atractylodes japonica reduced cyclooxygenase-2 (COX-2), iNOS at 900 μg/ml concentration. 6. Hot water extracts of Atractylodes japonica reduced cyclooxygenase-2 (COX-2) at 900 μg/ml, iNOS at 300 μg/ml concentration.

Conclusion : Methanol and hot water extracts of Atractylodes japonica has strongly implied that it's possible for it to be a food through food processing, in addition it was expected that isolation and purification test would bring out more various clinical effects.