골유도 재생술을 위한 PLLA/키토산 차단막의 제조 및 특성 분석

Abstract

Recently, the implant surgery literature has reported on a variety of implant procedures designed to regenerate lost bone tissue. To place implants at their ideal sites rather than on existing bone tissue, the regeneration of lost bone tissue is required, and diverse procedures have been developed to achieve this result. Guided bone regeneration (GBR) and guided tissue regeneration (GTR) are based on the dual concepts of excluding undesirable tissues from areas of defect and selectively enabling the access of healthy tissue required for regeneration. Membranes used for GBR or GTR should exhibit biocompatibility, histocompatibility, cell blocking capacity, nutrient-carrying capacity, and clinical ease of manipulation, among other properties. Membranes should be placed securely in the bone defect areas, and they should have a functional surface that can prevent the proliferation of connective tissue cells. The purpose of this study was to improve the biological, physical, and chemical properties of GBR membranes. New types of GBR membranes were fabricated to use poly(L-lactic acid) (PLLA) and chitosan via the solution casting method. PLLA has been used to prepare scaffolds to guide tissue regeneration in the field of tissue engineering research. However, one of the limitations of PLLA as an ideal biomaterial is its high hydrophobicity. To improve the hydrophilicity of PLLA, PLLA/Chitosan GBR membranes were enhanced with introducing NH2 groups to surfaces via plasma polymerization. Themorphology and components of the membranes were characterized by scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and Attenuated Total Reflectance - Fourier transform infrared spectroscopy (ATR-FTIR). The hydrophilicity of the membranes was examined by measuring their water contact angles. MG-63 cells were cultured on each sample and cell viability was examined using an MTT assay. The addition of Chitosan and NH₂ treated membranes ensured improvements in hydrophilicity and cell viability. These results suggested that the membrane may be useful in guided bone regeneration (GBR).