고정화 효소를 이용한 alpha-ketoglutarate 생산공정 개발

Abstract

α-Ketoglutarate (also called oxo-glutarate) is an important nitrogen acceptor in metabolism. It is produced by oxidative deamination of glutamate or reductive carboxylation of succinate. It has been used for the production of creatin-α-ketoglutarate which used as a fitness supplement. In this study, we developed a noble biotransformation reactor for α-ketoglutarate production using enzyme aggregated nanofiber reactor, micro reactor, monolith reactor and high pressure reactor for the development of biotransformation reactor. L-glutamic dehydrogenase was aggregated on the surface of nanofiber and used. The conversions of a micro reactor, monolith reactor and high pressure reactor were 20.1%, 20.3% and 53.0% respectively