사람 두경부 편평세포암종 FaDu 세포에서 Angelica decursiva 추출물에 의한 세포성장 억제기전

Abstract

The Angelica decursiva has been used in Korean traditional medicine as an antitussive, an analgesic, an antipyretic and a cough remedy. However, the anti-cancer activity of Angelica decursiva has not yet been well defined. In this study, therefore, the cytotoxic activity of ethanol extract of Angelica decursiva root (EEAD) and the mechanism of cell death exhibited by EEAD were examined in FaDu human head and neck squamous cell carcinoma. The cytotoxic effect of EEAD on cell growth in FaDu cells was examined using MTT assay. In addition, the cell death mechanism by EEAD was examined using DNA fragmentation analysis, immunoblotting and caspase activation measurement. Treatment of EEAD in FaDu cells induced the apoptotic cell death in a concentration- and a time-dependent manner as determined by MTT assay and DNA fragmentation analysis. Furthermore, the proteolytic processing of caspase-3, -7 and -9 was increased by EEAD treatment in FaDu cells. In addition, activation of caspase-3 and -7 was detected in living FaDu cells by fluorescence microscopy. These results suggest that EEAD can induce the suppression of cell growth and cell apoptosis in FaDu cells, and therefore, that it may have potential properties for anti-cancer drug discovery.