배양 상아질모세포 분화에 미치는 키토산의 효과

Abstract

Dentin is a mineralized tissue formed by odontoblasts that are differentiated from ectomesenchymal cells. The molecular mechanism of differentiation in odontoblasts remains unclear. Chitosan is the N-deacetylated derivative of chitin and has been recommended as a functional material because of its biocompatibility, biodegradability, non-toxicity and adsorption properties. Several bioactivities such as antitumor activity, cholesterol-lowering effects, immunoenhancing effects, antidiabetic effects, wound healing effects, antifungal activities and antimicrobial activities of chitosan also have been known. Although numerous literatures are available on the pharmacological and biological activities, the biological activity of chitosan in tooth formation process has not yet been well defined. In this study, the effect of chitosan was, therefore, investigated in the differentiation of MDPC-23 cells derived from mouse dental papilla cells. To determine the characteristic alteration of MDPC-23 cells by chitosan in the differentiation of MDPC-23 cells, it was examined by RT-PCR, observation of cell morphology and Alizaline red-S staining after inducing experimental differentiation in MDPC-23 cells. The results are as follows.

1. Chitosan significantly increased and accelerated the expression of ALP mRNA as compared with the control in a time-dependent manner in the differentiation of MDPC-23 cells. 2. Chitosan significantly accelerated the decrease of ColⅠ mRNA expression as compared with the control in a time-dependent manner in the differentiation of MDPC-23 cells. 3. Chitosan significantly accelerated the decrease of ON mRNA expression as compared with the control in a time-dependent manner in the differentiation of MDPC-23 cells. 4. Chitosan significantly accelerated the mineralization as compared with the control culture in the differentiation of MDPC-23 cells.

These results suggest that the chitosan facilitate the odontoblast ddifferentiation and mineralization in the differentiation of MDPC-23 cells, and therefore, that it may have potential properties for development and its clinical application of dentin regeneration materials.