레이져다이오드 조사에 의한 흰 쥐 피부상처 치유 촉진에 관한 연구

Abstract

Background: Laser diode irradiation has some beneficial effects on the wound healing. I performed preliminary animal study to get basic data of laser diode irradiation, because there is no proved genetic and biochemical mechanism. Methods: 8 months old male rat (NTacSam:SD, SamtakoBioKorea), weighting 250 ~ 300 g were used. Round blade, which has 1 cm of diameter, was penetrated through the subcutaneous level after elevating the skin just above the thoracic spine. Laser diode of 655 nm, 785 nm and 850 nm were irradiated to the skin wound for 9 days, 20 minutes in a day. 8 rats were used in 4 group including non-irradiated group. Complete blood count was calculated. Pan-Cytokeratin, Actin and Masson's trichrome staining was carried to evaluate the cellular and protein component relating wound healing. Wound size was measured at 9 th postoperative day with computer system. Result: Collagen formation was graded as 2+, 3+, 4+ in the order of non-radiation group, 655 nm and 785 nm groups and 850 nm group respectively. Myofibroblast, which has important role in wound healing, were formed more abundantly in laser diode irradiated group than non-irradiated group. The mean value of PCNA were 67.8±5.0, 84.0±4.6, 78.0±6.8 and 74.2±4.0 nm in the order of non-radiation group, 655 nm, 785 nm groups and 850 nm group respectively. Mean value of defect size were 2,840±124 um, 1,689±125 um, 1,254±94 um, 1,423±113 in the order of non-radiation group, 655 nm and 785 nm groups and 850 nm group respectively. Conclusion: Laser diode irradiation has beneficial effect in the formation of fibroblast and collagen formation and result in better wound healing.