TGF-β1에 의해 유도되는 HSP27이 생쥐 치유두 유래 MDPC-23 세포 이동에 미치는 역할

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Introduction: Transforming growth factor β1 (TGFβ1) regulates cellular function including cell growth, differentiation, angiogenesis, migration and metastasis. However, signal transduction pathways of TGF-β1 are mostly undefined in mouse dental papilla derived MDPC-23 cells. In this study, we investigated TGFβ1-induced migration focusing on heat shock protein 27 (Hsp27) activation.
Methods: Cellular response by TGFβ1 in MDPC-23 cells was measured by Western blot and immunocytochemistry assay. Cell migration was determined by counting migrated cells using Chemotaxis Cell Migration Assay kit.
Results: TGFβ1 induced cell migration and increased the phosphorylation of Hsp27 and p38 MAPK in MDPC-23 cells. However, TGF-β1 did not affect Akt/NF-κB signaling to regulate migration of MDPC-23cells. Inhibiting p38 MAPK with SB203580 blocked TGFβ1-induced Hsp27 activation and cell migration.
Conclusion: Hsp27 phosphorylation followed by p38 MAPK activation was required for TGFβ1-induced migration and Hsp27 itself contributed to MDPC-23 cell migration.
Alternative Title
Transforming growth factor β1-induced heat shock protein 27 promotes migration of mouse dental papilla derived MDPC-23 cells
Alternative Author(s)
Kwon Seong Min
일반대학원 치의공학과
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Table Of Contents
목 차 ······························································································ ⅰ
도목차 ······················································································ ⅲ
ABSTRACT ·················································································· ⅳ

Ⅰ. 서 론 ························································································ 1

Ⅱ. 재료 및 방법 ············································································ 4
1. 재료 및 항체 ············································································· 4
2. 세포 배양 ·················································································· 4
3. MTT 분석 ················································································· 4
4. 세포 이동 분석 ·········································································· 5
5. Western blot 분석 ····································································· 5
6. 자료 분석 ···················································································· 6

Ⅲ. 결과 ··························································································· 7
1. TGF-β1이 MDPC-23 세포 증식에 미치는 영향 ·················· 7
2. TGF-β1이 MDPC-23 세포 이동에 미치는 영향 ················· 8
3. TGF-β1이 p38 MAPK/Hsp27의 인산화에 미치는 영향 ····· 9
4. TGF-β1이 integrin β1/Akt의 활성화에 미치는 영향 ········ 10
5. Hsp27활성 억제가 TGF-β1에 의해 유도되는 MDPC-23 세포 이동에 미치는 영향 ·································································· 12
6. TGF-β1에 의해 활성화된 p38 MAPK가 Hsp27의 인산화에 미치는 영향···················································································· 14
7. p38 MAPK 저해제가 TGF-β1에 의해 유도된 MDPC-23 세포의 이동에 미치는 영향 ······························································ 16
8. TGF-β1에 의해 유도된 MDPC-23의 세포 이동 기전 모델 ············································································································· 17

Ⅳ. 고찰 ························································································· 18

V. 참고문헌 ····················································································· 21
조선대학교 일반대학원
권성민. (2010). TGF-β1에 의해 유도되는 HSP27이 생쥐 치유두 유래 MDPC-23 세포 이동에 미치는 역할.
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General Graduate School > 3. Theses(Master)
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