티타늄 표면에 히알루론산과 카르복실 메틸 키토산의 고정화가 MG-63 세포에 미치는 영향

Abstract

Plasma surface modification can be used to improve the surface properties of titanium, by creating functional groups to produce bioactive materials and different surface topography. The natural polymer chitosan and Hyaluronic acid (HA) are component of extracellular matrix (ECM) and influence on several celluar events such as attachment and proliferation. These bioactive materials are non-toxic and biodegradable, and also it has been reported several biological. In this study, the biocompatible titanium surface was developed by plasma polymerization of allylamine (AA) and consequently grafting carboxymethyl chitosan (CMCH) and then, HA was immobilized with covalent bond on the modified substrates in the presence of 1-ethyl-3-3-dimethylaminopropyl carbodiimide (EDC). All surface modified samples were characterized by the attenuated total reflectance-fourier transform infrared (ATR-FTIR) and x-ray photoelectron spectroscopy (XPS). The surface topology of specimen was investigated by scanning electron microscope (SEM) and atomic force microscope (AFM). In vitro studies wereperformed with L929 mouse fibroblast cell to investigate the effect of biological change on fibroblast cell behavior. The cell viability and proliferation were investigated by the MTT assay and the results revealed that cell adhesion and proliferation were enhanced.