줄기세포의 분화능의 기원에 따른 비교 - 견봉하 점액낭, 골수, 탯줄 혈액

Abstract

Introduction: Evaluation of differential potential of stem cells is mandatory for the better understanding of their character and function. We compared the differential potential of stem cells from three different sources for detecting their functional capacities Meterials and Methods: Our study involved three stem cell sources-subacromial bursal tissue, bone marrow, and umbilical cord-derived stem cells. Under consent we obtain subacromial bursa tissue and bone marrow from the patient having operation. After procuring, we applied specific induction media for neurogenic, adipogenic and osteogenic differentiation and flow-cytometry analysis was done to reveal the cell surface antigen. Results: We obtained all 8 neural and 8 adipogenic, but 5 of 8 osseous differentiation among the subacromial bursal tissue origin group. Bone marrow derived cells showed differential potential in all 6 neural, 5 adipogenic, but 4 of 5 osseous. Umbilical cord blood derived cells revealed 65 of 67 neural, 29 of 54 adipogenic, 39 of 57 osseous differentiations. Conclusion: Mesenchymal stem cells are prospective object for the using cell therapy. These cells are characterized primarily by expression of surface markers and their differential potential. Our study with stem cell from subacromial bursal tissue, bone marrow and umbilical cord revealed that each stem cell has differential potential and unique function based on its origin. Varius stem cells show multi-lineage differentiations in vitro which can be correlated to in vivo conditions

Key Words: subacromial bursa, Bone marrow, Cord blood, Stem cell, Differential potential