미세교세포 활성을 유도하는 LPS와 Maganese에 대한 Curcumin과 Melatonin의 효과

Abstract

ABSTRACT

Effects of Curcumin and Melatonin on Lipopolysaccharide/Manganese-Induced Microglial Activation

Park Jeong Ae Advisor : Prof. Chun Hong Sung Department of Biotechnology Graduate School of Chosun University

Microglia activation induces inflammation mediators and causes neuroinflammation response which is directly related with various neurodegenerative diseases. In this study, the beneficial effects of curcumin and melatonin against LPS/MnCl2-induced microglial activation were investigated. Curcumin (1 M) and melatonin (10 M) effectively reduced lipid peroxidation induced by LPS (500 ng/ml)/MnCl2 (250 M) exposure. Furthermore, both curcumin and melatonin exerted the antioxidant effects by elevating and maintaining GSH activity which was significantly reduced by LPS/MnCl2. Moreover, curcumin and melatonin markedly attenuated LPS/MnCl2-stimulated inducible nitric oxide synthase (iNOS) mRNA and protein expression, as well as NO production and expression of proinflammatory cytokines including TNF-α and IL-1β in BV-2 miroglial cells. Both curcumin and melatonin also showed inhibition of LPS/MnCl2-induced IκB-α phosphorylation and subsequent NF-κB activation in BV-2 cells. In addition, pretreatment of BV-2 cells with curcumin and melatonin dramatically inhibited LPS- and/or MnCl2-induced phagocytosis. These results suggested that both curcumin and melatonin are bioactive microglia regulators having anti-inflammatory functions.