다낭성난소 유발 흰쥐의 피부상처 회복에 따른 분비백혈구단백분해효소억제제의 발현

Abstract

ABSTRACT

Expression of Secretory leukocyte protease inhibitor (SLPI) on the rat skin wound healing in Polycystic ovary syndrome (PCOS)

Kim Sung Sin Advisor : Prof. Jang Hyun-Sun D.D.S, M.S.D., PhD. Department of Dental Science, Graduate School of Chosun University

Estrogen promotes the wound healing rate through the reduction of excessive inflammatory response, accelerating re-epithelialization and increasing amount of collagen deposition. Excessive administration of Estradiol valerate (EV) which using for hormonal therapy was known that decreased abruptly concentration of estrogen and induce the Polycystic ovary syndrome (PCOS). Secretory leukocyte protease inhibitor (SLPI) promotes wound healing through the inhibition of excessive inflammatory response and acceleration of keratinocyte proliferation. The expression of SLPI by stimulation with estrogen has been reported to increase. The purpose of this study is to investigate functional role of SLPI on the cutaneous wound healing process within PCOS as the systemic reduction of estrogen concentration. The wound area of PCOS group was larger than the normal group. Keratinocyte migration rate of PCOS group was lower than the normal group. The inflammatory cells in wound area within PCOS group were increased compared to the normal group and collagen deposition was more increased on normal group. The Mac-3 positive cells were increased in wound area of PCOS group compared with normal group at day 1 but increased on normal group at day3 and day 5. SLPI mRNA and protein expression in skin wound were higher on PCOS group than normal group. Expression intensity of SLPI in dermis was more increased in PCOS group compared with normal group at day 3 and day 5 but in case of regenerated epithelium, intensity was high in normal group. Taken together, skin wound healing was delayed in PCOS group compared with normal group. In unwounded skin of PCOS group, expression of SLPI was lower than normal group. After wounding, the expression of SLPI was increased in wounded skin of PCOS group compared with normal group. In conclusion, SLPI may be an important regulatory molecule involved in the skin wounds within PCOS, which has hormonal imbalance with reduced estrogen. Specially, we suggest that SLPI can be possibly used as an agent for reducing of side effects by hormonal treatment on the chronic inflammation and wound healing process of the postmenopausal women.

Key Words: Estrogen, Wound healing, Estradiol valerate, Polycystic ovary syndrome, Secretory leukocyte protease inhibitor